flgE

168

flagellar hook protein

locus: BSU_16290

Molecular weight: 27.32 kDa

pI: 4.73

Protein length: 264 aa

Gene length: 795 bp

function: movement and chemotaxis

product: flagellar hook protein

essential: no

synonyms: flgE, flgG

List of homologs in different organisms, belongs to COG4786 (Galperin et al., 2021)

Gene

Coordinates: 1,700,182 → 1,700,976

Phenotypes of a mutant

• not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation [Pubmed|26122431]
• defective in [category|SW.4.1.4|Swarming] motility [pubmed|35638827]

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The protein

Protein family

• [wiki|Flagella basal body rod proteins family] (according to UniProt)

Structure

• [PDB|3A69] (from ''Salmonella enterica'', 44% identity) [Pubmed|19913483]
• [AF|P23446]

Paralogous protein(s)

• [protein|13F35BC7EE8215D7A92A639BEB18647AC276D215|flhP], [protein|8EFE091CFB89FC406CD60776977C9C302C7826E0|flhO]

[wiki|Localization]

• bacterial flagellum basal body (according to UniProt)
• extracellular (no signal peptide) [Pubmed|18957862]
• membrane associated [Pubmed|18763711]

Biological materials

Mutant

• DS4681 (marker-less  in NCIB3610) [Pubmed|22730131]
• BKE16290 (Δ[gene|DC906ED8D787602B5796BAD8FD81F02C4BAC8D13|flgE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16290 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAATATATTCCCCCAGAT,  downstream forward: _UP4_TAAGGAGGGAGGGGAGGCGA
• BKK16290 (Δ[gene|DC906ED8D787602B5796BAD8FD81F02C4BAC8D13|flgE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16290 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAATATATTCCCCCAGAT,  downstream forward: _UP4_TAAGGAGGGAGGGGAGGCGA

References

Reviews

• 26490009

Original Publications

• 26122431,14651647,18957862,18763711,9657996,8157612,15175317,17850253,24386445,22730131,19913483,30201778