Difference between revisions of "FhuD"

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|style="background:#ABCDEF;" align="center"|'''Function''' || siderophore uptake  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || siderophore uptake  
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU33320 fhuD]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33320 fhuD]
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/FhuD FhuD]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/FhuD FhuD]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fhuB]]'', ''[[yvsH]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fhuB]]'', ''[[yvsH]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15338&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU33320 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU33320 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU33320 Advanced_DNA]
 
|-
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:fhuD_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:fhuD_context.gif]]

Revision as of 13:50, 13 May 2013

  • Description: hydroxamate siderophore ABC transporter (only ferrichrome) (binding protein)

Gene name fhuD
Synonyms
Essential no
Product hydroxamate siderophore ABC transporter
(only ferrichrome) (binding protein)
Function siderophore uptake
Gene expression levels in SubtiExpress: fhuD
Interactions involving this protein in SubtInteract: FhuD
Metabolic function and regulation of this protein in SubtiPathways:
metal ion homeostasis
MW, pI 34 kDa, 9.158
Gene length, protein length 945 bp, 315 aa
Immediate neighbours fhuB, yvsH
Sequences Protein DNA Advanced_DNA
Genetic context
FhuD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FhuD expression.png















Categories containing this gene/protein

ABC transporters, acquisition of iron, iron metabolism

This gene is a member of the following regulons

Fur regulon

The gene

Basic information

  • Locus tag: BSU33320

Phenotypes of a mutant

  • poor growth with the xenosiderophore ferrichrome as single source of iron PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: bacterial solute-binding protein 8 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cell membrane (according to Swiss-Prot), extracellular (signal peptide) PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: fhuD (according to DBTBS)
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Marcus Miethke, Timo Kraushaar, Mohamed A Marahiel
Uptake of xenosiderophores in Bacillus subtilis occurs with high affinity and enhances the folding stabilities of substrate binding proteins.
FEBS Lett: 2013, 587(2);206-13
[PubMed:23220087] [WorldCat.org] [DOI] (I p)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Juliane Ollinger, Kyung-Bok Song, Haike Antelmann, Michael Hecker, John D Helmann
Role of the Fur regulon in iron transport in Bacillus subtilis.
J Bacteriol: 2006, 188(10);3664-73
[PubMed:16672620] [WorldCat.org] [DOI] (P p)

Noel Baichoo, Tao Wang, Rick Ye, John D Helmann
Global analysis of the Bacillus subtilis Fur regulon and the iron starvation stimulon.
Mol Microbiol: 2002, 45(6);1613-29
[PubMed:12354229] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)