Fbp

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  • Description: fructose-1,6-bisphosphatase, involved in gluconeogenesis

Gene name fbp
Synonyms fdp, yydE
Essential no
Product fructose-1,6-bisphosphatase
Function gluconeogenesis
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism, Sugar catabolism
MW, pI 77 kDa, 5.601
Gene length, protein length 2013 bp, 671 aa
Immediate neighbours yydF, yydD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Fbp context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU40190

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate (according to Swiss-Prot)
  • Protein family: FBPase class 3 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 3D1R (from E. coli, 49% identity, 68% similarity)PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Greg Brown, Alexander Singer, Vladimir V Lunin, Michael Proudfoot, Tatiana Skarina, Robert Flick, Samvel Kochinyan, Ruslan Sanishvili, Andrzej Joachimiak, Aled M Edwards, Alexei Savchenko, Alexander F Yakunin
Structural and biochemical characterization of the type II fructose-1,6-bisphosphatase GlpX from Escherichia coli.
J Biol Chem: 2009, 284(6);3784-92
[PubMed:19073594] [WorldCat.org] [DOI] (P p)

Y Fujita, K Yoshida, Y Miwa, N Yanai, E Nagakawa, Y Kasahara
Identification and expression of the Bacillus subtilis fructose-1, 6-bisphosphatase gene (fbp).
J Bacteriol: 1998, 180(16);4309-13
[PubMed:9696785] [WorldCat.org] [DOI] (P p)

Y Fujita, E Freese
Isolation and properties of a Bacillus subtilis mutant unable to produce fructose-bisphosphatase.
J Bacteriol: 1981, 145(2);760-7
[PubMed:6257649] [WorldCat.org] [DOI] (P p)

Y Fujita, E Freese
Purification and properties of fructose-1,6-bisphosphatase of Bacillus subtilis.
J Biol Chem: 1979, 254(12);5340-9
[PubMed:221467] [WorldCat.org] (P p)