FbaA

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  • Description: fructose 1,6-bisphosphate aldolase, glycolytic/ gluconeogenic enzyme

Gene name fbaA
Synonyms fba, fba1, tsr
Essential no
Product fructose-1,6-bisphosphate aldolase
Function enzyme in glycolysis/ gluconeogenesis
Gene expression levels in SubtiExpress: fbaA
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism, Sugar catabolism
MW, pI 30,2 kDa, 5.03
Gene length, protein length 855 bp, 285 amino acids
Immediate neighbours ywjH, spo0F
Sequences Protein DNA Advanced_DNA
Genetic context
FbaA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FbaA expression.png
















Categories containing this gene/protein

carbon core metabolism, phosphoproteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU37120

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate (according to Swiss-Prot)
  • Protein family: class II fructose-bisphosphate aldolase family (according to Swiss-Prot)
  • Paralogous protein(s): FbaB

Extended information on the protein

  • Kinetic information: Reversible Michaelis-Menten PubMed
  • Domains:
    • 2 x Dihydroxyacetone phosphate binding domain (210–212), (231–234)
  • Modification: phosphorylation on Thr-212 and Thr-234 PubMed
  • Cofactor(s): Zn2+ (Metalloenzyme)
  • Effectors of protein activity:
    • Inhibited by alpha-ketoglutarate, oxaloacetate and pyruvate PubMed PubMed
    • Activated by NH4+ PubMed

Database entries

  • Structure: 3Q94 (from Bacillus anthracis)
  • KEGG entry: [3]

Additional information

  • Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution
  • extensive information on the structure and enzymatic properties of FbaA can be found at Proteopedia

Expression and regulation

  • Regulation:
    • constitutively expressed PubMed
    • strongly repressed in response to glucose starvation in M9 medium PubMed
  • Regulatory mechanism:
  • Additional information:
    • The mRNA has a long 5' leader region. This may indicate RNA-based regulation PubMed

Biological materials

  • Expression vector:
    • for expression in B. subtilis, in pBQ200: pGP1423, available in Jörg Stülke's lab
    • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP88, available in Jörg Stülke's lab
    • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP395, available in Jörg Stülke's lab
  • lacZ fusion: pGP601 (in pAC6)
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References