Difference between revisions of "FbaA"

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Revision as of 13:06, 12 January 2009

  • Description: Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis

Gene name fbaA
Synonyms fba, fba1, tsr
Essential yes
Product fructose-1,6-bisphosphate aldolase
Function aldol condensation & reverse reaction
MW, pI 30,2 kDa, 5.03
Gene length, protein length 855 bp, 285 amino acids
Immediate neighbours spo0F, ywjH
Gene sequence (+200bp) Protein sequence
Genetic context
File:GenE context.gif












The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate
  • Protein family: class II fructose-bisphosphate aldolase family.
  • Paralogous protein(s): FbaB

Extended information on the protein

  • Kinetic information:
  • Domains:
    • 2 x Dihydroxyacetone phosphate binding domain (210–212), (231–234)
  • Modification: Phosphorylated during sporulation
  • Cofactor(s): 2 x zinc ion
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry: [3]
  • KEGG entry: [4]
  • E.C. number: [5]

Additional information

Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution

Expression and regulation

  • Operon: fbaA, fbaA-ywjH
  • Sigma factor:
  • Regulation: constitutively expressed
  • Regulatory mechanism:
  • Additional information:

Biological materials

Labs working on this gene/protein

Your additional remarks

References

  1. Trach K, Chapman JW & Piggot P (1988) Complete sequence and transcriptional analysis of the spo0F region of the Bacillus subtilis chromosome J Bacteriol. 170: 4194-4208. PubMed
  2. Ludwig H, Homuth G & Schmalisch M (2001) Transcription of glycolytic genes and operons in Bacillus subtilis: evidence for the presence of multiple levels of control of the gapA operon Mol Microbiol. 41: 409-22. PubMed