FbaA

• Description: fructose 1,6-bisphosphate aldolase, glycolytic/ gluconeogenic enzyme
  
  

Gene name	fbaA
Synonyms	fba, fba1, tsr
Essential	yes
Product	fructose-1,6-bisphosphate aldolase
Function	enzyme in glycolysis/ gluconeogenesis
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism, Sugar catabolism
MW, pI	30,2 kDa, 5.03
Gene length, protein length	855 bp, 285 amino acids
Immediate neighbours	ywjH, spo0F
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU37120

Phenotypes of a mutant

• Essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate (according to Swiss-Prot)

• Protein family: class II fructose-bisphosphate aldolase family (according to Swiss-Prot)
• Paralogous protein(s): FbaB

Extended information on the protein

• Kinetic information: Reversible Michaelis-Menten PubMed

• Domains:
  • 2 x Dihydroxyacetone phosphate binding domain (210–212), (231–234)

• Modification: phosphorylation on Thr-212 and Thr-234 PubMed

• Cofactor(s): Zn2+ (Metalloenzyme)

• Effectors of protein activity:
  • Inhibited by alpha-ketoglutarate, oxaloacetate and pyruvate PubMed PubMed
  • Activated by NH4+ PubMed

• Interactions:

• Localization:

Database entries

• Structure: 2FJK (from Thermus caldophilus) PubMed

• UniProt: P13243

• KEGG entry: [3]

• E.C. number: 4.1.2.13

Additional information

Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution

Expression and regulation

• Operon:
  • fbaA PubMed
  • fbaA-ywjH PubMed

• Sigma factor:

• Regulation: constitutively expressed PubMed

• Regulatory mechanism:

• Additional information:
  • The mRNA has a long 5' leader region. This may indicate RNA-based regulation PubMed

Biological materials

• Mutant:
• GP591 (fbaA::cat), available in Stülke lab
• GP596 (fbaA::erm), available in Stülke lab

• Expression vector:
  • for expression in B. subtilis, in pBQ200: pGP1423, available in Stülke lab
  • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP88, available in Stülke lab
  • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP395, available in Stülke lab

• lacZ fusion: pGP601 (in pAC6)

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody: