fadN

168

3-hydroxyacyl-CoA dehydrogenase (acetoacetyl-CoA)

locus: BSU_32840

Molecular weight: 89.96 kDa

pI: 6.53

Protein length: 815 aa

Gene length: 2448 bp

function: fatty acid degradation

product: 3-hydroxyacyl-CoA dehydrogenase (acetoacetyl-CoA)

essential: no

ec: 1.1.1.35

synonyms: fadN, yusL

List of homologs in different organisms, belongs to COG1250 (Galperin et al., 2021)

Gene

Coordinates: 3,370,025  3,372,472

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The protein

Catalyzed reaction/ biological activity

• (3S)-hydroxyacyl-CoA + NAD⁺ --> 3-oxoacyl-CoA + H⁺ + NADH (according to UniProt)

Protein family

• 3-hydroxyacyl-CoA dehydrogenase family (with [protein|FB95ABDC8557F26525E8B211595C130A7203ABC0|mmgB], according to UniProt)

[wiki|Cofactors]

• FMN or FAD [Pubmed|21635694]

Structure

• [AF|O32178]

Biological materials

Mutant

• MGNA-A604 (yusL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/604 NBRP B. subtilis, Japan]
• BKE32840 ([gene|4787161A022E938A6D58A505D92369F889C7DE04|fadN]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32840 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAGATATCCCCCTTGAA,  downstream forward: _UP4_CCTTTACGGAATTAGGAGGG
• BKK32840 ([gene|4787161A022E938A6D58A505D92369F889C7DE04|fadN]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32840 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAGATATCCCCCTTGAA,  downstream forward: _UP4_CCTTTACGGAATTAGGAGGG

References

• 12817086,12850135,17189250,17919287,23033921,21398533,31113899