fadM

168

fatty acid degradation, similar to proline dehydrogenase

locus: BSU_32850

Molecular weight: 34.30 kDa

pI: 9.38

Protein length: 302 aa

Gene length: 909 bp

function: fatty acid degradation

product: unknown

essential: no

synonyms: fadM, yusM

List of homologs in different organisms, belongs to COG0506 (Galperin et al., 2021)

Gene

Coordinates: 3,372,740 → 3,373,648

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The protein

Catalyzed reaction/ biological activity

• quinone + L-proline --> (S)-1-pyrroline-5-carboxylate + quinol + H⁺ (according to UniProt)

Protein family

• proline dehydrogenase family (with [protein|1F548515402950572E7212901729B2480432D1B9|putB], according to UniProt)

[wiki|Cofactors]

• FAD (according to UniProt)

Structure

• [PDB|4H6Q] (from Deinococcus radiodurans, 48% identity) [pubmed|23151026]
• [AF|O32179]

Paralogous protein(s)

• [protein|1F548515402950572E7212901729B2480432D1B9|putB]

Biological materials

Mutant

• MGNA-A598 (yusM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/598 NBRP B. subtilis, Japan]
• BKE32850 (Δ[gene|F8C51A86268DC6B2152B139D2D0B25CC3D69A5D5|fadM]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32850 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCGACTCCCTCCCGCCG,  downstream forward: _UP4_TGAAAGCAGCCGTGGGAAGC
• BKK32850 (Δ[gene|F8C51A86268DC6B2152B139D2D0B25CC3D69A5D5|fadM]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32850 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCGACTCCCTCCCGCCG,  downstream forward: _UP4_TGAAAGCAGCCGTGGGAAGC

References

• 17189250,12817086,23151026