Difference between revisions of "FadB"
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=Expression and regulation= | =Expression and regulation= | ||
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* '''Operon:''' | * '''Operon:''' | ||
| + | ** ''[[lcfA]]-[[fadR]]-[[fadB]]-[[etfB]]-[[etfA]]'' {{PubMed|17189250}} | ||
| + | ** ''[[fadR]]-[[fadB]]-[[etfB]]-[[etfA]]'' {{PubMed|17189250}} | ||
* '''[[Sigma factor]]:''' | * '''[[Sigma factor]]:''' | ||
| + | ** ''[[fadR]]'': [[SigA]] {{PubMed|17189250}} | ||
| + | |||
| + | * '''Regulation:''' | ||
| + | ** induced by long-chain fatty acids ([[FadR]]) {{PubMed|17189250}} | ||
| + | ** repressed by glucose (3.8-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed] | ||
| + | |||
| + | * '''Regulatory mechanism:''' | ||
| + | ** [[FadR]]: transcription repression {{PubMed|17189250}} | ||
| − | * '''Regulation:''' | + | * '''Regulation:''' |
** repressed in the absence of long-chain fatty acids ([[FadR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/17189250 PubMed] | ** repressed in the absence of long-chain fatty acids ([[FadR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/17189250 PubMed] | ||
| + | ** [[CcpA]]: transcription repression | ||
| − | + | * '''Additional information:''' | |
| − | |||
| − | |||
| − | * '''Additional information:''' | ||
=Biological materials = | =Biological materials = | ||
Revision as of 21:26, 15 December 2009
- Description: 3-hydroxyacyl-CoA dehydratase
| Gene name | fadB |
| Synonyms | ysiB |
| Essential | no |
| Product | 3-hydroxyacyl-CoA dehydratase |
| Function | fatty acid degradation |
| MW, pI | 27 kDa, 4.951 |
| Gene length, protein length | 774 bp, 258 aa |
| Immediate neighbours | etfB, fadR |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU28540
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O (according to Swiss-Prot)
- Protein family: enoyl-CoA hydratase/isomerase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: P94549
- KEGG entry: [3]
- E.C. number: 4.2.1.17
Additional information
Expression and regulation
- Regulation:
- Regulation:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Hiroshi Matsuoka, Kazutake Hirooka, Yasutaro Fujita
Organization and function of the YsiA regulon of Bacillus subtilis involved in fatty acid degradation.
J Biol Chem: 2007, 282(8);5180-94
[PubMed:17189250]
[WorldCat.org]
[DOI]
(P p)
Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135]
[WorldCat.org]
[DOI]
(P p)
