Difference between revisions of "FabI"

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(Extended information on the protein)
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** inhibited by triclosan {{PubMed|11325930}}
 
** inhibited by triclosan {{PubMed|11325930}}
  
* '''Interactions:'''
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* '''[[SubtInteract|Interactions]]:'''  
  
* '''Localization:'''
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=== Database entries ===
 
=== Database entries ===

Revision as of 19:40, 11 August 2011

  • Description: enoyl-acyl carrier protein reductase

Gene name fabI
Synonyms yjbW
Essential no
Product enoyl-acyl carrier protein reductase
Function fatty acid biosynthesis
Metabolic function and regulation of this protein in SubtiPathways:
Lipid synthesis
MW, pI 27 kDa, 5.605
Gene length, protein length 774 bp, 258 aa
Immediate neighbours thiD, cotO
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
FabI context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

biosynthesis of lipids

This gene is a member of the following regulons

FapR regulon

The gene

Basic information

  • Locus tag: BSU11720

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Acyl-[acyl-carrier-protein] + NAD+ = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH (according to Swiss-Prot)
  • Protein family: FabI subfamily (according to Swiss-Prot)
  • Paralogous protein(s): FabL, one of the two proteins has to be present for viability PubMed

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • inhibited by triclosan PubMed

Database entries

  • Structure: 3OIF (complex with NAD and inhibitor triclosan) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed when the cells experience a lack of malonyl-CoA (FapR) PubMed
    • induced upon fatty acid biosynthesis inhibition PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Yasutaro Fujita, Hiroshi Matsuoka, Kazutake Hirooka
Regulation of fatty acid metabolism in bacteria.
Mol Microbiol: 2007, 66(4);829-39
[PubMed:17919287] [WorldCat.org] [DOI] (P p)

Stephen W White, Jie Zheng, Yong-Mei Zhang, Rock
The structural biology of type II fatty acid biosynthesis.
Annu Rev Biochem: 2005, 74;791-831
[PubMed:15952903] [WorldCat.org] [DOI] (P p)

Original Publications