fabI

168

enoyl-acyl carrier protein reductase

locus: BSU_11720

Molecular weight: 27.72 kDa

pI: 5.61

Protein length: 258 aa

Gene length: 777 bp

function: fatty acid biosynthesis

product: enoyl-acyl carrier protein reductase

essential: no

ec: 1.3.1.9

synonyms: fabI, yjbW

List of homologs in different organisms, belongs to COG0623 (Galperin et al., 2021)

Gene

Coordinates: 1,247,788 1,248,564

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The protein

Catalyzed reaction/ biological activity

• 2,3-saturated acyl-[ACP] + NAD⁺ --> (2E)-enoyl-[ACP] + H⁺ + NADH (according to UniProt)

Protein family

• [wiki|Short-chain dehydrogenases/reductases (SDR) family] (according to UniProt)

Structure

• [PDB|3OIF] (complex with NAD and inhibitor triclosan) [Pubmed|21185310]
• [AF|P54616]

Effectors of protein activity

• inhibited by triclosan [Pubmed|11325930]

Paralogous protein(s)

• [protein|5964B6E817260DA7937796DDFA753A665A04D650|fabL], one of the two proteins has to be present for viability [Pubmed|17114254]

Additional information

• belongs to the 100 most abundant proteins [PubMed|15378759]

Biological materials

Mutant

• MGNA-B168 (yjbW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1167 NBRP B. subtilis, Japan]
• BKE11720 ([gene|60A519E4C1485B9F5BF36E83EDE884B396857CB4|fabI]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGTATAAAGCCTCCTA, downstream forward: _UP4_TAAGCAGGATCTATATCAAG
• BKK11720 ([gene|60A519E4C1485B9F5BF36E83EDE884B396857CB4|fabI]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGTATAAAGCCTCCTA, downstream forward: _UP4_TAAGCAGGATCTATATCAAG

References

Reviews

• 15952903,17919287

Original Publications

• 12737802,17114254,8953047,11007778,21185310,21383089,15378759,29914988,31113899