fabHA

168

beta-ketoacyl-acyl carrier protein synthase III, principal condensing enzyme responsible for the initiation of fatty acid synthesis in non-stressed B. subtilis cells

locus: BSU_11330

Molecular weight: 33.58 kDa

pI: 5.05

Protein length: 312 aa

Gene length: 939 bp

function: fatty acid biosynthesis

product: beta-ketoacyl-acyl carrier protein synthase III

essential: no

ec: 2.3.1.180

synonyms: fabHA, yjaX

List of homologs in different organisms, belongs to COG0332 (Galperin et al., 2021)

Gene

Coordinates: 1,208,222  1,209,160

Phenotypes of a mutant

• significant increase in the proportion of straight-chain fatty acids with a concomitant increase in 31:0-carbon phosphatidylethanolamine species [Pubmed|21542858]

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The protein

Catalyzed reaction/ biological activity

• acetyl-CoA + H⁺ + malonyl-[ACP] --> 3-oxobutanoyl-[ACP] + CO₂ + CoA (according to UniProt)

Protein family

• [wiki|thiolase-like superfamily] (according to UniProt)

Structure

• [PDB|8VD9] [Pubmed|38310992]
• [PDB|8VDA] (complex with CoA) [Pubmed|38310992]
• [AF|O34746]

Paralogous protein(s)

• [protein|FC818B3A8A3080231C1F8DE95377325C8E754DCE|fabHB], one of the two proteins has to be present for viability [Pubmed|17114254]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Additional information

• affinity for butyryl-CoA, but prefers acetyl-CoA in fatty acid biosynthesis [Pubmed|19820084]

Biological materials

Mutant

• MGNA-B145 (yjaX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1144 NBRP B. subtilis, Japan]
• BKE11330 ([gene|40E064365588CFED483FBFED02BFC0DA531CCC4A|fabHA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11330 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTAGGGAAGACTCCTTTA,  downstream forward: _UP4_TAAAAAAAGGTGAGGTGCAC
• BKK11330 ([gene|40E064365588CFED483FBFED02BFC0DA531CCC4A|fabHA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11330 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTAGGGAAGACTCCTTTA,  downstream forward: _UP4_TAAAAAAAGGTGAGGTGCAC

References

Reviews

• 15952903,17919287

Original Publications

• 12737802,17114254,10629181,10673437,19820084,21383089,21542858,25196128,30925337,31113899,38310992