ExoA

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  • Description: apurinic/apyrimidinic endonuclease, multifunctional DNA-repair enzyme, important for spore dormance

Gene name exoA
Synonyms
Essential no
Product apurinic/apyrimidinic endonuclease
Function repair of oxidative DNA damage in spores
Gene expression levels in SubtiExpress: exoA
MW, pI 29 kDa, 4.592
Gene length, protein length 756 bp, 252 aa
Immediate neighbours ccpB, rpsR
Sequences Protein DNA DNA_with_flanks
Genetic context
ExoA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ExoA expression.png















Categories containing this gene/protein

DNA repair/ recombination, sporulation proteins

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

  • Locus tag: BSU40880

Phenotypes of a mutant

  • an exoA nfo double mutant is impaired in germination and spore outgrowth due to the accumulation of DNA lesions, this can be rescued by inactivation of disA PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • ExoA is functionally redundant with Nfo

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates (according to Swiss-Prot)
  • Protein family: DNA repair enzymes AP/exoA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed late during sporulation in the forespore (SigG) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

Original publications