Difference between revisions of "ExoA"

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= [[Categories]] containing this gene/protein =
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{{SubtiWiki category|[[DNA repair/ recombination]]}},
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{{SubtiWiki category|[[sporulation proteins]]}}
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= This gene is a member of the following [[regulons]] =
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{{SubtiWiki regulon|[[SigG regulon]]}}
  
 
=The gene=
 
=The gene=
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= Categories containing this gene/protein =
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{{SubtiWiki category|[[DNA repair/ recombination]]}},
 
{{SubtiWiki category|[[sporulation proteins]]}}
 
 
=The protein=
 
=The protein=
  

Revision as of 01:21, 9 December 2010

  • Description: apurinic/apyrimidinic endonuclease, multifunctional DNA-repair enzyme, important for spore dormance

Gene name exoA
Synonyms
Essential no
Product apurinic/apyrimidinic endonuclease
Function repair of oxidative DNA damage in spores
MW, pI 29 kDa, 4.592
Gene length, protein length 756 bp, 252 aa
Immediate neighbours ccpB, rpsR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ExoA context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

DNA repair/ recombination, sporulation proteins

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

  • Locus tag: BSU40880

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates (according to Swiss-Prot)
  • Protein family: DNA repair enzymes AP/exoA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed late during sporulation in the forespore (SigG) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Marcelo Barraza-Salas, Juan R Ibarra-Rodríguez, Silvia J Mellado, José M Salas-Pacheco, Peter Setlow, Mario Pedraza-Reyes
Effects of forespore-specific overexpression of apurinic/apyrimidinic endonuclease Nfo on the DNA-damage resistance properties of Bacillus subtilis spores.
FEMS Microbiol Lett: 2010, 302(2);159-65
[PubMed:19930460] [WorldCat.org] [DOI] (I p)

Juan R Ibarra, Alma D Orozco, Juan A Rojas, Karina López, Peter Setlow, Ronald E Yasbin, Mario Pedraza-Reyes
Role of the Nfo and ExoA apurinic/apyrimidinic endonucleases in repair of DNA damage during outgrowth of Bacillus subtilis spores.
J Bacteriol: 2008, 190(6);2031-8
[PubMed:18203828] [WorldCat.org] [DOI] (I p)

José M Salas-Pacheco, Barbara Setlow, Peter Setlow, Mario Pedraza-Reyes
Role of the Nfo (YqfS) and ExoA apurinic/apyrimidinic endonucleases in protecting Bacillus subtilis spores from DNA damage.
J Bacteriol: 2005, 187(21);7374-81
[PubMed:16237020] [WorldCat.org] [DOI] (P p)

T Shida, T Ogawa, N Ogasawara, J Sekiguchi
Characterization of Bacillus subtilis ExoA protein: a multifunctional DNA-repair enzyme similar to the Escherichia coli exonuclease III.
Biosci Biotechnol Biochem: 1999, 63(9);1528-34
[PubMed:10540738] [WorldCat.org] [DOI] (P p)