Difference between revisions of "Era"

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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
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=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=
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* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
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** [[Era]]-[[16S rRNA]] {{PubMed|19706445}}
  
 
* '''[[Localization]]:'''
 
* '''[[Localization]]:'''
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* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU25290&redirect=T BSU25290]
 
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU25290&redirect=T BSU25290]
  
* '''Structure:'''
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* '''Structure:''' [http://pdb.org/pdb/explore/explore.do?structureId=3IEV 3IEV] ([[Era]] from ''Aquifex aeolicus'', 50% identity/ 77% similarity, complex with the 3' end of [[16S rRNA]]) {{PubMed|19706445}}
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P42182 P42182]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P42182 P42182]
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==Original publications==
 
==Original publications==
<pubmed>12427945, 6330116 </pubmed>
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<pubmed>12427945, 6330116 19706445</pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 11:47, 25 February 2015

  • Description: GTP-binding protein, involved in the assembly of the 30S ribosomal subunit

Gene name era
Synonyms bex, yqfH
Essential yes PubMed
Product GTP-binding protein
Function ribosome assembly
Gene expression levels in SubtiExpress: era
MW, pI 33 kDa, 8.376
Gene length, protein length 903 bp, 301 aa
Immediate neighbours yqzL, cdd
Sequences Protein DNA DNA_with_flanks
Genetic context
Era context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Era expression.png















Categories containing this gene/protein

translation, essential genes, GTP-binding proteins

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

  • Locus tag: BSU25290

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • Protein family: Era subfamily (according to Swiss-Prot) Era/Obg family

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3IEV (Era from Aquifex aeolicus, 50% identity/ 77% similarity, complex with the 3' end of 16S rRNA) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP837, available in Stülke lab
    • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP845, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

Your additional remarks

References

Reviews

Natalie Verstraeten, Maarten Fauvart, Wim Versées, Jan Michiels
The universally conserved prokaryotic GTPases.
Microbiol Mol Biol Rev: 2011, 75(3);507-42, second and third pages of table of contents
[PubMed:21885683] [WorldCat.org] [DOI] (I p)

Robert A Britton
Role of GTPases in bacterial ribosome assembly.
Annu Rev Microbiol: 2009, 63;155-76
[PubMed:19575570] [WorldCat.org] [DOI] (I p)


Original publications

Chao Tu, Xiaomei Zhou, Joseph E Tropea, Brian P Austin, David S Waugh, Donald L Court, Xinhua Ji
Structure of ERA in complex with the 3' end of 16S rRNA: implications for ribosome biogenesis.
Proc Natl Acad Sci U S A: 2009, 106(35);14843-8
[PubMed:19706445] [WorldCat.org] [DOI] (I p)

Takuya Morimoto, Pek Chin Loh, Tomohiro Hirai, Kei Asai, Kazuo Kobayashi, Shigeki Moriya, Naotake Ogasawara
Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis.
Microbiology (Reading): 2002, 148(Pt 11);3539-3552
[PubMed:12427945] [WorldCat.org] [DOI] (P p)

P Z Wang, R H Doi
Overlapping promoters transcribed by bacillus subtilis sigma 55 and sigma 37 RNA polymerase holoenzymes during growth and stationary phases.
J Biol Chem: 1984, 259(13);8619-25
[PubMed:6330116] [WorldCat.org] (P p)