Difference between revisions of "Era"

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Revision as of 13:57, 16 May 2013

  • Description: GTP-binding protein, involved in the assembly of the 30S ribosomal subunit

Gene name era
Synonyms bex, yqfH
Essential yes PubMed
Product GTP-binding protein
Function ribosome assembly
Gene expression levels in SubtiExpress: era
MW, pI 33 kDa, 8.376
Gene length, protein length 903 bp, 301 aa
Immediate neighbours yqzL, cdd
Sequences Protein DNA DNA_with_flanks
Genetic context
Era context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Era expression.png




























Categories containing this gene/protein

translation, essential genes, GTP-binding proteins

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

  • Locus tag: BSU25290

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • Protein family: Era subfamily (according to Swiss-Prot) Era/Obg family

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP837, available in Stülke lab
    • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP845, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

Your additional remarks

References

Reviews


Original publications

Takuya Morimoto, Pek Chin Loh, Tomohiro Hirai, Kei Asai, Kazuo Kobayashi, Shigeki Moriya, Naotake Ogasawara
Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis.
Microbiology (Reading): 2002, 148(Pt 11);3539-3552
[PubMed:12427945] [WorldCat.org] [DOI] (P p)

P Z Wang, R H Doi
Overlapping promoters transcribed by bacillus subtilis sigma 55 and sigma 37 RNA polymerase holoenzymes during growth and stationary phases.
J Biol Chem: 1984, 259(13);8619-25
[PubMed:6330116] [WorldCat.org] (P p)