Difference between revisions of "Era"

From SubtiWiki
Jump to: navigation, search
Line 22: Line 22:
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqzL]]'', ''[[cdd]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqzL]]'', ''[[cdd]]''
 
|-
 
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25290 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25290 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25290 Advanced_DNA]
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25290 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25290 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25290 DNA_with_flanks]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:era_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:era_context.gif]]

Revision as of 10:45, 14 May 2013

  • Description: GTP-binding protein, involved in the assembly of the 30S ribosomal subunit

Gene name era
Synonyms bex, yqfH
Essential yes PubMed
Product GTP-binding protein
Function ribosome assembly
Gene expression levels in SubtiExpress: era
MW, pI 33 kDa, 8.376
Gene length, protein length 903 bp, 301 aa
Immediate neighbours yqzL, cdd
Sequences Protein DNA DNA_with_flanks
Genetic context
Era context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Era expression.png




























Categories containing this gene/protein

translation, essential genes, GTP-binding proteins

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

  • Locus tag: BSU25290

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • Protein family: Era subfamily (according to Swiss-Prot) Era/Obg family

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP837, available in Stülke lab
    • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP845, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

Your additional remarks

References

Reviews

Natalie Verstraeten, Maarten Fauvart, Wim Versées, Jan Michiels
The universally conserved prokaryotic GTPases.
Microbiol Mol Biol Rev: 2011, 75(3);507-42, second and third pages of table of contents
[PubMed:21885683] [WorldCat.org] [DOI] (I p)

Robert A Britton
Role of GTPases in bacterial ribosome assembly.
Annu Rev Microbiol: 2009, 63;155-76
[PubMed:19575570] [WorldCat.org] [DOI] (I p)


Original publications

Takuya Morimoto, Pek Chin Loh, Tomohiro Hirai, Kei Asai, Kazuo Kobayashi, Shigeki Moriya, Naotake Ogasawara
Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis.
Microbiology (Reading): 2002, 148(Pt 11);3539-3552
[PubMed:12427945] [WorldCat.org] [DOI] (P p)

P Z Wang, R H Doi
Overlapping promoters transcribed by bacillus subtilis sigma 55 and sigma 37 RNA polymerase holoenzymes during growth and stationary phases.
J Biol Chem: 1984, 259(13);8619-25
[PubMed:6330116] [WorldCat.org] (P p)