Difference between revisions of "Era"

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= [[Categories]] containing this gene/protein =
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{{SubtiWiki category|[[translation]]}},
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{{SubtiWiki category|[[essential genes]]}},
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{{SubtiWiki category|[[GTP-binding proteins]]}}
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= This gene is a member of the following [[regulons]] =
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{{SubtiWiki regulon|[[SigB regulon]]}}
  
 
=The gene=
 
=The gene=
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= Categories containing this gene/protein =
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{{SubtiWiki category|[[translation]]}},
 
{{SubtiWiki category|[[essential genes]]}},
 
{{SubtiWiki category|[[GTP-binding proteins]]}}
 
 
=The protein=
 
=The protein=
  

Revision as of 21:29, 8 December 2010

  • Description: GTP-binding protein, involved in the assembly of the 30S ribosomal subunit

Gene name era
Synonyms bex, yqfH
Essential yes PubMed
Product GTP-binding protein
Function ribosome assembly
MW, pI 33 kDa, 8.376
Gene length, protein length 903 bp, 301 aa
Immediate neighbours recO, cdd
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Era context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

translation, essential genes, GTP-binding proteins

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

  • Locus tag: BSU25290

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • Protein family: Era subfamily (according to Swiss-Prot) Era/Obg family

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP837, available in Stülke lab
    • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP845, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

Your additional remarks

References

Reviews

Robert A Britton
Role of GTPases in bacterial ribosome assembly.
Annu Rev Microbiol: 2009, 63;155-76
[PubMed:19575570] [WorldCat.org] [DOI] (I p)

Original publications

Takuya Morimoto, Pek Chin Loh, Tomohiro Hirai, Kei Asai, Kazuo Kobayashi, Shigeki Moriya, Naotake Ogasawara
Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis.
Microbiology (Reading): 2002, 148(Pt 11);3539-3552
[PubMed:12427945] [WorldCat.org] [DOI] (P p)

P Z Wang, R H Doi
Overlapping promoters transcribed by bacillus subtilis sigma 55 and sigma 37 RNA polymerase holoenzymes during growth and stationary phases.
J Biol Chem: 1984, 259(13);8619-25
[PubMed:6330116] [WorldCat.org] (P p)