Difference between revisions of "Era"

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(Biological materials)
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* '''Mutant:'''
 
* '''Mutant:'''
  
* '''Expression vector:''' pGP93 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab
+
* '''Expression vector:'''  
       
+
** for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [[SPINE]], in [[pGP380]]: pGP837, available in [[Stülke]] lab
 +
** for expression/ purification from ''E. coli'' with N-terminal His-tag, in [[pHW844]]: pGP845, available in [[Stülke]] lab
 +
   
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
  

Revision as of 18:06, 1 September 2009

  • Description: GTP-binding protein

Gene name era
Synonyms bex, yqfH
Essential yes PubMed
Product GTP-binding protein
Function
MW, pI 33 kDa, 8.376
Gene length, protein length 903 bp, 301 aa
Immediate neighbours recO, cdd
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Era context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU25290

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • Protein family: Era subfamily (according to Swiss-Prot) Era/Obg family

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP837, available in Stülke lab
    • for expression/ purification from E. coli with N-terminal His-tag, in pHW844: pGP845, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

Your additional remarks

References