epsC

168

UDP-N-acetylglucosamine 4,6-dehydratase, required for extracellular polysaccharide synthesis, this gene is inactive in B. subtilis 168

locus: BSU_34350

Molecular weight: 66.09 kDa

pI: 8.77

Protein length: 598 aa

Gene length: 1797 bp

function: [category|SW.4.1.2|Biofilm formation], biosynthesis of UDP-N,N’-diacetylbacillosamine (with [protein|0D24AB8D446CFFBC6DEAD3C10D1FA59551545FE8|epsN] and [protein|40EA584CF77FFF7D7E4E74B414EC69ADE3A1585B|epsM])

product: UDP-N-acetylglucosamine 4,6-dehydratase

essential: no

synonyms: epsC, yveM

List of homologs in different organisms, belongs to COG1086 (Galperin et al., 2021)

Gene

Coordinates: 3,526,407  3,528,203

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The protein

Catalyzed reaction/ biological activity

• UDP-N-acetylglucosamine (UDP-GlcNAc) --> UDP-2,6-dideoxy-2-acetamido 4-keto glucose (UDP-4-keto) [pubmed|30222950]

Protein family

• [wiki|Polysaccharide synthase family] (according to UniProt)

[wiki|Cofactors]

• NAD⁺ [pubmed|30222950]

Structure

• [PDB|5BJW] (from Campylobacter jejuni, corresponds to the C-terminal part of EpsC, aa 242 ... 571, 47% identity) [pubmed|29053280]
• [AF|P71052]

Paralogous protein(s)

• [protein|F42B27D5D7F3F39828940E377ABCC0730B25EA88|yodU], (for SpsM)
  [protein|240CD6EA3793821F5109252BDEF69C0120E454EF|ypqP], (for SpsM)

[wiki|Localization]

• cell membrane (according to Swiss-Prot)

Biological materials

Mutant

• MGNA-A071 (yveM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/71 NBRP B. subtilis, Japan]
• BKE34350 ([gene|5DB168A3D087AAEB003D7548A1A4356ABD07F712|epsC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34350 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGACAGTCTTCTCCGGT,  downstream forward: _UP4_AGCGTTCATTAGGGGGAGTG
• BKK34350 ([gene|5DB168A3D087AAEB003D7548A1A4356ABD07F712|epsC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34350 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGACAGTCTTCTCCGGT,  downstream forward: _UP4_AGCGTTCATTAGGGGGAGTG

References

Reviews

• 20384681,20735481

Research papers

• 27655338,30222950,29053280

The EAR [wiki|RNA switch]

• 20374491,20230605

Labs working on this gene/protein

• [wiki|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]