epsB

168

extracellular polysaccharide synthesis, protein tyrosine kinase, phosphorylation of [protein|5D717F9F54693FD0AA8BC49E10348637858F88B9|epsE]

locus: BSU_34360

Molecular weight: 24.53 kDa

pI: 9.92

Protein length: 227 aa

Gene length: 684 bp

function: biofilm formation

product: protein tyrosine kinase

essential: no

synonyms: epsB, yveL

List of homologs in different organisms, belongs to COG0489 (Galperin et al., 2021)

Gene

Coordinates: 3,528,462  3,529,145

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The protein

Catalyzed reaction/ biological activity

• phosphorylation of [protein|5D717F9F54693FD0AA8BC49E10348637858F88B9|epsE], to stimulate [protein|5D717F9F54693FD0AA8BC49E10348637858F88B9|epsE] activity and biofilm formation [pubmed|25085422]
• ATP + L-tyrosyl-[protein] --> ADP + H⁺ + O-phospho-L-tyrosyl-[protein] (according to UniProt)

Protein family

• BY kinase, see the [http://bykdb.ibcp.fr/BYKdb/BYKdbHelp Bacterial Protein Tyrosine Kinase Database]
• CpsD/CapB family (with [protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA], according to UniProt)

Structure

• [PDB|2VED] (CapB, the homolog in ''Staphylococcus aureus'') [Pubmed|18547145]
• [AF|P71051]

Modification

• autophosphorylated on Tyr-225 and Tyr-227 [Pubmed|25085422]

Paralogous protein(s)

• [protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA]

Biological materials

Mutant

• MGNA-A072 (yveL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/72 NBRP B. subtilis, Japan]
• GP1518 (aphA3) [Pubmed|24493247], available in [wiki|Jörg Stülke]'s lab
• GP1519 (''[gene|F6C4634BF871D9A01E27014FFED7527C54BF560D|epsA]-[gene|9B668909E1380B287ACE58561DCF45FC29184D8B|epsB]'', aphA3) [Pubmed|24493247], available in [wiki|Jörg Stülke]'s lab
• BKE34360 ([gene|9B668909E1380B287ACE58561DCF45FC29184D8B|epsB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34360 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CTTTTTTCTAAAGATCACTC,  downstream forward: _UP4_TAGTTTTTGTAAAGGTGATG
• BKK34360 ([gene|9B668909E1380B287ACE58561DCF45FC29184D8B|epsB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34360 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CTTTTTTCTAAAGATCACTC,  downstream forward: _UP4_TAGTTTTTGTAAAGGTGATG

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]) [Pubmed|24493247], available in [wiki|Jörg Stülke]'s lab

FLAG-tag construct

• GP1541 epsB-FLAG 3x spc (based on [wiki|pGP1331]) available in [wiki|Jörg Stülke]'s lab

labs

• [wiki|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]

References

Reviews

• 20735481,24554699,25540643

Original Publications

• 15661000,16430695,18047568,18647168,18547145,20817675,21856853,21815947,23646920,24493247,24728941,25085422