epsA

168

extracellular polysaccharide synthesis, putative transmembrane modulator of [protein|9B668909E1380B287ACE58561DCF45FC29184D8B|epsB] activity, might activate [protein|9B668909E1380B287ACE58561DCF45FC29184D8B|epsB] autophosphorylation and substrate phosphorylation

locus: BSU_34370

Molecular weight: 25.75 kDa

pI: 6.07

Protein length: 234 aa

Gene length: 705 bp

function: biofilm formation

product: unknown

essential: no

synonyms: epsA, yveK

List of homologs in different organisms, belongs to COG3944 (Galperin et al., 2021)

Gene

Coordinates: 3,529,151 → 3,529,855

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The protein

Protein family

• CapA family (with [protein|A696434A086A428D411CAF45B37CD8F82AC2503F|capA] and [protein|8DCA50478136628DD9E5D01E89609A686EED9F57|tkmA], according to UniProt)
• CpsC/CapA family (with [protein|8DCA50478136628DD9E5D01E89609A686EED9F57|tkmA], according to UniProt)

Structure

• [AF|P71050]

Paralogous protein(s)

• [protein|8DCA50478136628DD9E5D01E89609A686EED9F57|tkmA]

[wiki|Localization]

• cell membrane (according to UniProt)

Biological materials

Mutant

• MGNA-A073 (yveK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/73 NBRP B. subtilis, Japan]
• GP1517 (aphA3) [Pubmed|24493247], available in [wiki| Jörg Stülke]'s lab
• GP1519 (''[gene|F6C4634BF871D9A01E27014FFED7527C54BF560D|epsA]-[gene|9B668909E1380B287ACE58561DCF45FC29184D8B|epsB]'', aphA3) [Pubmed|24493247], available in [wiki| Jörg Stülke]'s lab
• GP1567 ''[gene|F6C4634BF871D9A01E27014FFED7527C54BF560D|epsA]''::aphA3 ''[gene|8DCA50478136628DD9E5D01E89609A686EED9F57|tkmA]''::spc [Pubmed|24493247], available in [wiki| Jörg Stülke]'s lab
• BKE34370 (Δ[gene|F6C4634BF871D9A01E27014FFED7527C54BF560D|epsA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34370 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTATTCATAGCCTTCAG,  downstream forward: _UP4_AAACATTTCGGGGAGTGAAG
• BKK34370 (Δ[gene|F6C4634BF871D9A01E27014FFED7527C54BF560D|epsA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34370 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTATTCATAGCCTTCAG,  downstream forward: _UP4_AAACATTTCGGGGAGTGAAG

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]) [Pubmed|24493247], available in [wiki| Jörg Stülke]'s lab

FLAG-tag construct

• GP1526 epsA-FLAG 3x spc (based on [wiki|pGP1331]) [Pubmed|24493247], available in [wiki| Jörg Stülke]'s lab

Antibody

• **

GFP fusion

• GP1569 epsA-gfp (spc), available in [wiki| Jörg Stülke]'s lab

labs

• [wiki|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]

References

Reviews

• 20735481

Original Publications

• 15661000,16430695,18047568,18647168,20351052,20817675,21856853,21815947,23646920,24493247,25085422,26283769,32483306