eno
168
enolase, glycolytic/ gluconeogenic enzyme, universally conserved protein
locus
BSU_33900
Molecular weight
46.42 kDa
pI
4.49
function
enzyme in glycolysis/ gluconeogenesis
product
enolase
essential
Yes
ec
4.2.1.11
synonyms
eno
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0148 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,476,555 3,477,847
Phenotypes of a mutant
no growth on LB, requires glucose and malate [Pubmed|23420519]
essential according to Kobayashi et al. on LB [Pubmed|12682299]
Advertisement
The protein
Catalyzed reaction/ biological activity
(2R)-2-phosphoglycerate --> H2O + phosphoenolpyruvate (according to UniProt)
Protein family
Enolase family (single member, according to UniProt)
[wiki|Domains]
substrate binding domain (366369)
[wiki|Cofactors]
Mg2+
Structure
[PDB|4A3R] [Pubmed|22198292]
[PDB|1W6T] (from ''Streptococcus pneumoniae'') [Pubmed|15476816]
[AF|P37869]
Modification
phosphorylation on Thr-141 AND Ser-259 AND Tyr-281 AND Ser-325 [Pubmed|17218307,16493705,17726680]
Effectors of protein activity
Inhibited by EDTA [Pubmed|25885]
Kinetic information
reversible Michaelis-Menten [Pubmed|25885]
[wiki|Localization]
cytoplasm [Pubmed|16479537,27708634]
membrane associated [Pubmed|18763711]
aggregates at the cel poles of some cells [pubmed|29439991]
exported, this requires a long, unbent helix (from A108 to L126) [Pubmed|15003462,21856851]
Additional information
Enolase is a [wiki|moonlighting proteins|moonlighting protein] [Pubmed|19193632]
There are indications that this enzyme is an octamer [Pubmed|25885]
extensive information on the structure and enzymatic properties of Eno can be found at [http://www.proteopedia.org/wiki/index.php/Enolase Proteopedia]
belongs to the 100 most abundant proteins [PubMed|15378759]
Biological materials
Mutant
GP594 (''eno''::''cat''), available in [wiki|Jörg Stülke]'s lab, [Pubmed|23420519]
GP698 (''eno''-''[gene|C86FB523451AC172A20801829B717E5A5F914211|pgm]''::''cat''), available in [wiki|Jörg Stülke]'s lab, [Pubmed|23420519]
Expression vectors
pGP1426 (expression of ''[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]'' in ''B. subtilis'', in [wiki|pBQ200]), available in [wiki|Jörg Stülke]'s lab
pGP1500 (expression of ''[gene|C86FB523451AC172A20801829B717E5A5F914211|pgm]'' and ''[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]'' in ''B. subtilis'', in [wiki|pBQ200]), available in [wiki|Jörg Stülke]'s lab
pGP563 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab, [pubmed|20389117]
pGP1276 (N-terminal Strep-tag, purification from ''E. coli'', in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
pGP93 (N-terminal Strep-tag, purification from ''B. subtilis'', for [wiki|SPINE], in [wiki|pGP380]), available in [wiki|Jörg Stülke]'s lab, [pubmed|19193632]
GP1215 (chromosomal ''eno''-''Strep'' fusion, ''spc''), purification from ''B. subtilis'', for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab, [pubmed|19193632]
FLAG-tag construct
GP1214 (spc, based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab [pubmed|21803996]
lacZ fusion
see [gene|ECF0F2E906BF94F509817752827CA189AFBE53FE|pgk]
GFP fusion
pHT315-yfp-eno, available in [wiki|Mijakovic] lab
GP1700 (in [wiki|pBP43]), expression of '' eno-GFP''::''spc'' under the native promoter, available in [wiki|Jörg Stülke]'s lab [Pubmed|27708634]
labs
[wiki|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
References
Reviews
Original Publications
Subcellular localization of enolase
Page visits: 12012
Time of last update: 2024-04-24 11:27:43
Author of last update: Jstuelk