ecsA

168

[wiki|ABC transporter] (ATP-binding protein), mutation affects proteolysis by [protein|CB50289535EA537F63BADD459BD11AA7759A6658|rasP] (no processing of [protein|D6C2B8ABBBD1F9F2D36C34098D5B811A15C60D3B|rsgI] resulting in absence if [protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI] activity, [protein|4E720917045032E8B45CB8AF6E5C13AE0E48EE33|rsiW], and [protein|79712F4CF884A660C71B45B3397AEFA5C0AFA683|ftsL])

locus: BSU_10040

Molecular weight: 27.57 kDa

pI: 5.64

Protein length: 247 aa

Gene length: 744 bp

function: regulation of the secretion apparatus and of intra-membrane proteolysis

product: [wiki|ABC transporter] (ATP-binding protein)

essential: no

synonyms: ecsA, prsT, ecs-26, yhaD

List of homologs in different organisms, belongs to COG1131 (Galperin et al., 2021)

Gene

Coordinates: 1,077,440  1,078,183

Phenotypes of a mutant

• no processing of [protein|4E720917045032E8B45CB8AF6E5C13AE0E48EE33|rsiW] and [protein|79712F4CF884A660C71B45B3397AEFA5C0AFA683|ftsL] [Pubmed|18599827]
• inactivation of ''[gene|59A7B26F810D7FEB07C176A8B2ECF6B83803DE49|ecsA]'' reduces sporulation efficiency to 0.1% that of wild type cells [Pubmed|26735940]
• a [gene|4F9D70C4BB4FCA809BEAC341C3F8122FB7FD8872|cwlO] [gene|59A7B26F810D7FEB07C176A8B2ECF6B83803DE49|ecsA] mutant is not viable, due to lack of expression of [gene|321F248C22D7283C0F3323F1F4069E36F8D7FE6C|lytE] [Pubmed|36265902]
• [gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA] [gene|59A7B26F810D7FEB07C176A8B2ECF6B83803DE49|ecsA] double mutants have a growth defect that can be suppressed by reduced fatty acid synthesis (mutations in [gene|22B3039DF4D106F121DBC765F49878C9B324FDB2|accA], [gene|ADD1CE87C1C5BA44FFF5499EA5E155C4C53A1C22|accB], [gene|1DFFEB155028A5C6B4B344769EF7DAAC14983322|accC], [gene|E4997526CC534E38413030E90F7A135ECF65DF40|accD] or downregulation of the [wiki|FapR regulon] due to specific mutations in [gene|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]) [pubmed|37017514]

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The protein

Protein family

• [wiki|ABC transporter superfamily] (according to UniProt)

[wiki|Domains]

• [wiki|ABC transporter domain] (aa 4-234) (according to UniProt)

Structure

• [PDB|4RVC] (from Geobacillus kaustophilus, 71% identity) [pubmed|25724946]
• [AF|P55339]

Paralogous protein(s)

• [protein|47C1A154AF6F67CA507A9F4FEA9576E7131EFB6D|ythP]

[wiki|Localization]

• membrane associated (via [protein|665BE3614B466067D64D1FB275ECDDE12CBFC9D4|ecsB]) [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|59A7B26F810D7FEB07C176A8B2ECF6B83803DE49|ecsA]-[gene|665BE3614B466067D64D1FB275ECDDE12CBFC9D4|ecsB]-[gene|6D07C98A82C8960133BC50E40EF5D56A21071316|ecsC]

  description: [pubmed|22383849]

additional information

• the mRNA is very stable (> 15 min) [pubmed|12884008]

Biological materials

Mutant

• BKE10040 ([gene|59A7B26F810D7FEB07C176A8B2ECF6B83803DE49|ecsA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10040 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGTTTCTCCCCTTATAG,  downstream forward: _UP4_GAGCTTACAAAGGAAGACGC
• BKK10040 ([gene|59A7B26F810D7FEB07C176A8B2ECF6B83803DE49|ecsA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10040 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGTTTCTCCCCTTATAG,  downstream forward: _UP4_GAGCTTACAAAGGAAGACGC

References

Reviews

• 29343670

Original Publications

• 8581172,12884008,10092453,18599827,10027970,26735940,25724946,36265902,37017514,36815589