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| − | * '''Description:''' inhibitor of [[AbrB]] activity<br/><br/>
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| − | {| align="right" border="1" cellpadding="2"
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| − | |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| − | |''abbA ''
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| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ykzF''
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| − | |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| − | |style="background:#ABCDEF;" align="center"| '''Product''' || anti-repressor
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| − | |style="background:#ABCDEF;" align="center"|'''Function''' || inhibition of [[AbrB]]
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| − | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 7 kDa, 4.642
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| − | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 195 bp, 65 aa
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| − | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykuK]]'', ''[[ykuL]]''
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| − | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13285]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| − | |colspan="2" | '''Genetic context''' <br/> [[Image:ykzF_context.gif]]
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| − | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| − | |}
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| − | __TOC__
| + | [[File:Example.jpg]] |
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| − | <br/><br/>
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| − | =The gene=
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| − | === Basic information ===
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| − | * '''Locus tag:''' BSU14120
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| − | ===Phenotypes of a mutant ===
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| − | === Database entries ===
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| − | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ykuJK-ykzF-ykuL-ccpC.html]
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| − | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13334]
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| − | === Additional information===
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| − | = Categories containing this gene/protein =
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| − | {{SubtiWiki category|[[transcription factors and their control]]}}
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| − | =The protein=
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| − | === Basic information/ Evolution ===
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| − | * '''Catalyzed reaction/ biological activity:''' inhibition of AbrB activity [http://www.ncbi.nlm.nih.gov/sites/entrez/18840696 PubMed]
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| − | * '''Protein family:'''
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| − | * '''Paralogous protein(s):'''
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| − | === Extended information on the protein ===
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| − | * '''Kinetic information:'''
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| − | * '''Domains:'''
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| − | * '''Modification:'''
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| − | * '''Cofactor(s):'''
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| − | * '''Effectors of protein activity:'''
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| − | * '''Interactions:''' [[AbbA]]-[[AbrB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18840696 PubMed]
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| − | * '''Localization:'''
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| − | === Database entries ===
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| − | * '''Structure:'''
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| − | * '''UniProt:''' [http://www.uniprot.org/uniprot/O31697 O31697]
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| − | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU14120]
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| − | * '''E.C. number:'''
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| − | === Additional information===
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| − | =Expression and regulation=
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| − | * '''Operon:''' ''[[ykuJ]]-[[ykuK]]-[[abbA]]-[[ykuL]]-[[ccpC]]'' {{PubMed|16395550}}
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| − | * '''Sigma factor:'''
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| − | * '''Regulation:'''
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| − | ** repressed by glucose (4.3-fold) ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed]
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| − | ** expressed under conditions that trigger sporulation ([[Spo0A]]) {{PubMed|18840696,14651647}}
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| − | * '''Regulatory mechanism:'''
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| − | ** [[CcpA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/12850135 PubMed]
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| − | ** [[Spo0A]]: transcription activation by [[Spo0A]]~P {{PubMed|18840696,14651647}}
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| − | * '''Additional information:'''
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| − | =Biological materials =
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| − | * '''Mutant:''' GP214 (deletion of ''[[ykuJ]]-[[ykuK]]-[[abbA]]-[[ykuL]]'', replaced by ''aphA3''), available in the [[Stülke]] lab
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| − | * '''Expression vector:'''
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| − |
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| − | * '''lacZ fusion:''' pGP708 (in [[pAC5]]), available in [[Stülke]] lab, pGP712 (in [[pAC6]]), available in [[Stülke]] lab
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| − | * '''GFP fusion:'''
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| − | * '''two-hybrid system:'''
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| − | * '''Antibody:'''
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| − | =Labs working on this gene/protein=
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| − | [[Richard Losick]], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]
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| − | =Your additional remarks=
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| − | =References=
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| − | <pubmed>,18840696,14651647,12850135 18840696 12850135,16395550 </pubmed>
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| − | [[Category:Protein-coding genes]]
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