DtpT

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  • Description: proton-dependent di- and tripeptide transporter

Gene name yclF
Synonyms dtpT
Essential no
Product peptide transporter
Function uptake of di- and tripeptides
Gene expression levels in SubtiExpress: yclF
MW, pI 53 kDa, 9.92
Gene length, protein length 1476 bp, 492 aa
Immediate neighbours yclE, yclG
Sequences Protein DNA DNA_with_flanks
Genetic context
YclF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YclF expression.png















Categories containing this gene/protein

transporters/ other, utilization of nitrogen sources other than amino acids, membrane proteins, sporulation proteins

This gene is a member of the following regulons

ScoC regulon

The gene

Basic information

  • Locus tag: BSU03670

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: POT family of secondary (PMF-dependent) transporters
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
    • 4IKV, the protein from Geobacillus kaustophilus (65% identity, 87% similarity), PubMed
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
    • repressed during exponential growth (ScoC) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Shintaro Doki, Hideaki E Kato, Nicolae Solcan, Masayo Iwaki, Michio Koyama, Motoyuki Hattori, Norihiko Iwase, Tomoya Tsukazaki, Yuji Sugita, Hideki Kandori, Simon Newstead, Ryuichiro Ishitani, Osamu Nureki
Structural basis for dynamic mechanism of proton-coupled symport by the peptide transporter POT.
Proc Natl Acad Sci U S A: 2013, 110(28);11343-8
[PubMed:23798427] [WorldCat.org] [DOI] (I p)

R Caldwell, R Sapolsky, W Weyler, R R Maile, S C Causey, E Ferrari
Correlation between Bacillus subtilis scoC phenotype and gene expression determined using microarrays for transcriptome analysis.
J Bacteriol: 2001, 183(24);7329-40
[PubMed:11717292] [WorldCat.org] [DOI] (P p)

E R Kunji, E J Smid, R Plapp, B Poolman, W N Konings
Di-tripeptides and oligopeptides are taken up via distinct transport mechanisms in Lactococcus lactis.
J Bacteriol: 1993, 175(7);2052-9
[PubMed:8458848] [WorldCat.org] [DOI] (P p)