drm

168

phosphopentomutase

locus: BSU_23500

Molecular weight: 43.77 kDa

pI: 4.97

Protein length: 394 aa

Gene length: 1185 bp

function: utilization of deoxyribose

product: phosphopentomutase

essential: no

ec: 5.4.2.7

synonyms: drm, yqkN

List of homologs in different organisms, belongs to COG1015 (Galperin et al., 2021)

Gene

Coordinates: 2,447,246  2,448,430

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The protein

Catalyzed reaction/ biological activity

• α-D-ribose 1-phosphate --> D-ribose 5-phosphate (according to UniProt)
• 2-deoxy-α-D-ribose 1-phosphate --> 2-deoxy-D-ribose 5-phosphate (according to UniProt)

Protein family

• phosphopentomutase family (single member, according to UniProt)

Structure

• [PDB|3M8W] (from ''B. cereus'', 77% identity, 86% similarity) [Pubmed|21193409]
• [AF|P46353]

Modification

• phosphorylation on (Thr-87 OR Thr-89) [Pubmed|17218307,17726680]
• CoA is attached to Cys-233 in spores [pubmed|33206970]

Biological materials

Mutant

• BKE23500 ([gene|9D07E43BF1FFB13CCEAC2B7AAA4EBDFB1079DD74|drm]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23500 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTTGAAAGCCTCCTTTT,  downstream forward: _UP4_TAGGGGGACTGTTTCTTGAA
• BKK23500 ([gene|9D07E43BF1FFB13CCEAC2B7AAA4EBDFB1079DD74|drm]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23500 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTTGAAAGCCTCCTTTT,  downstream forward: _UP4_TAGGGGGACTGTTTCTTGAA

References

• 36815589,10537218,17218307,17726680,22900538,33206970,35953809