Difference between revisions of "DnaN"
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{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"|'''Gene name''' | |style="background:#ABCDEF;" align="center"|'''Gene name''' | ||
|''dnaN'' | |''dnaN'' | ||
| − | |- | + | |- |
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''dnaG, dnaK '' | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''dnaG, dnaK '' | ||
| − | |- | + | |- |
| − | |||
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] | |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] | ||
| − | |- | + | |- |
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|style="background:#ABCDEF;" align="center"| '''Product''' || DNA polymerase III (gamma and tau subunits) | |style="background:#ABCDEF;" align="center"| '''Product''' || DNA polymerase III (gamma and tau subunits) | ||
| − | |- | + | |- |
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|style="background:#ABCDEF;" align="center"|'''Function''' || DNA replication | |style="background:#ABCDEF;" align="center"|'''Function''' || DNA replication | ||
| − | |- | + | |- |
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 41 kDa, 4.718 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 41 kDa, 4.718 | ||
| − | |- | + | |- |
| − | |||
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1134 bp, 378 aa | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1134 bp, 378 aa | ||
| − | |- | + | |- |
| − | |||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[dnaA]]'', ''[[yaaA]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[dnaA]]'', ''[[yaaA]]'' | ||
| − | |- | + | |- |
| − | + | |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/dnaN_nucleotide.txt Gene sequence (+200bp) ]''' | |
| − | |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni- | + | |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/dnaN_protein.txt Protein sequence]''' |
| − | + | |- | |
| − | |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni- | + | |colspan="2" | '''Genetic context''' <br/> [[Image:dnaN_context.gif]] |
| − | + | <div align="right"> <small>the origin of the image is [http://genolist.pasteur.fr/SubtiList/ SubtiList]<small>|- | |
| − | |- | ||
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| − | |colspan="2" | '''Genetic context''' <br/> [[Image: | ||
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|} | |} | ||
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* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| − | * '''Interactions:''' [[DnaA]]- | + | * '''Interactions:''' [[DnaA]]-[[YabA]]-[[DnaN]] [http://www.ncbi.nlm.nih.gov/sites/entrez/12060778 PubMed] |
| − | |||
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| − | * '''Localization:''' nucleoid (mid- | + | * '''Localization:''' nucleoid (mid-cell spot) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed] |
| − | |||
=== Database entries === | === Database entries === | ||
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* '''Swiss prot entry:''' | * '''Swiss prot entry:''' | ||
| − | * '''KEGG entry:''' [http://www.genome.jp/dbget- | + | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00020] |
| − | |||
* '''E.C. number:''' 2.7.7.7 | * '''E.C. number:''' 2.7.7.7 | ||
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' ''[[dnaA]]- | + | * '''Operon:''' ''[[dnaA]]-[[dnaN]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/2987848 PubMed] |
| − | |||
* '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/2987848 PubMed] | * '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/2987848 PubMed] | ||
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* '''GFP fusion:''' | * '''GFP fusion:''' | ||
| − | * '''two- | + | * '''two-hybrid system:''' |
| − | |||
* '''Antibody:''' | * '''Antibody:''' | ||
=Labs working on this gene/protein= | =Labs working on this gene/protein= | ||
| − | [[Philippe Noirot]], Jouy- | + | [[Philippe Noirot]], Jouy-en-Josas, France [http://locus.jouy.inra.fr/cms/index.php?id=18 homepage] |
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| − | |||
=Your additional remarks= | =Your additional remarks= | ||
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=References= | =References= | ||
| − | # Ogasawara et al. (1985) Structure and function of the region of the replication origin of the ''Bacillus subtilis'' chromosome. IV. Transcription of the oriC region and expression of DNA gyrase genes and other open reading frames.''Nucl. Acids Res.'' '''11:''' 2267- | + | # Ogasawara et al. (1985) Structure and function of the region of the replication origin of the ''Bacillus subtilis'' chromosome. IV. Transcription of the oriC region and expression of DNA gyrase genes and other open reading frames.''Nucl. Acids Res.'' '''11:''' 2267-2279. [http://www.ncbi.nlm.nih.gov/sites/entrez/2987848 PubMed] |
| − | + | # Fukuoka et al. (1990) Purification and characterization of an initiation protein for chromosomal replication, DnaA, in ''Bacillus subtilis''. ''J. Biochem.'' '''107:''' 732-739. [http://www.ncbi.nlm.nih.gov/sites/entrez/2168872 PubMed] | |
| − | # Fukuoka et al. (1990) Purification and characterization of an initiation protein for chromosomal replication, DnaA, in ''Bacillus subtilis''. ''J. Biochem.'' '''107:''' 732- | + | # Meile et al. (2006) Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory ''Proteomics'' '''6:''' 2135-2146. [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed] |
| − | + | # Molle et al. (2003) The Spo0A regulon of ''Bacillus subtilis''.''Mol. Microbiol.'' '''50:''' 1683-1701. [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed] | |
| − | # Meile et al. (2006) Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory ''Proteomics'' '''6:''' 2135- | + | # Noirot-Gros et al. (2002) An expanded view of bacterial DNA replication. ''Proc. Natl. Acad. Sci. USA'' '''99:''' 8342-8347. [http://www.ncbi.nlm.nih.gov/sites/entrez/12060778 PubMed] |
| − | + | # Noirot-Gros et al. (2002) Functional dissection of YabA, a negative regulator of DNA replication initiation in Bacillus Title ''Proc. Natl. Acad. Sci. USA'' '''103:''' 2368-2373. [http://www.ncbi.nlm.nih.gov/sites/entrez/16461910 PubMed] | |
| − | # Molle et al. (2003) The Spo0A regulon of ''Bacillus subtilis''.''Mol. Microbiol.'' '''50:''' 1683- | + | # Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed] |
| − | |||
| − | # Noirot- | ||
| − | |||
| − | |||
| − | # Noirot- | ||
| − | |||
| − | |||
| − | # Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page- | ||
| − | |||
Revision as of 16:06, 20 March 2009
- Description: DNA polymerase III (gamma and tau subunits)
| Gene name | dnaN |
| Synonyms | dnaG, dnaK |
| Essential | yes PubMed |
| Product | DNA polymerase III (gamma and tau subunits) |
| Function | DNA replication |
| MW, pI | 41 kDa, 4.718 |
| Gene length, protein length | 1134 bp, 378 aa |
| Immediate neighbours | dnaA, yaaA |
| Gene sequence (+200bp) | Protein sequence |
Genetic context 
the origin of the image is SubtiList|-
| |
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: nucleoid (mid-cell spot) PubMed
Database entries
- Structure:
- Swiss prot entry:
- KEGG entry: [3]
- E.C. number: 2.7.7.7
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Philippe Noirot, Jouy-en-Josas, France homepage
Your additional remarks
References
- Ogasawara et al. (1985) Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. IV. Transcription of the oriC region and expression of DNA gyrase genes and other open reading frames.Nucl. Acids Res. 11: 2267-2279. PubMed
- Fukuoka et al. (1990) Purification and characterization of an initiation protein for chromosomal replication, DnaA, in Bacillus subtilis. J. Biochem. 107: 732-739. PubMed
- Meile et al. (2006) Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory Proteomics 6: 2135-2146. PubMed
- Molle et al. (2003) The Spo0A regulon of Bacillus subtilis.Mol. Microbiol. 50: 1683-1701. PubMed
- Noirot-Gros et al. (2002) An expanded view of bacterial DNA replication. Proc. Natl. Acad. Sci. USA 99: 8342-8347. PubMed
- Noirot-Gros et al. (2002) Functional dissection of YabA, a negative regulator of DNA replication initiation in Bacillus Title Proc. Natl. Acad. Sci. USA 103: 2368-2373. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
