dnaG

dnaG
168

DNA primase, part of the [wiki|replisome]

locus
BSU_25210
Molecular weight
68.62 kDa
pI
6.71
Protein length
Gene length
function
[category|SW.3.1.1|DNA replication]
product
DNA primase
essential
yes
ec
2.7.7.-
synonyms
dnaG

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0358 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,601,528 → 2,603,339
Phenotypes of a mutant
essential [Pubmed|12682299]
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The protein
Catalyzed reaction/ biological activity
synthesis of RNA primers that initiate leading and lagging strand DNA synthesis
required for bacteriophage SPP1 replication [Pubmed|23268446]
Protein family
DNA primase family (single member, according to UniProt)
[wiki|Domains]
[wiki|TOPRIM domain] (aa 262-343) [Pubmed|9722641]
Structure
[PDB|1D0Q] (zinc binding domain, Geobacillus stearothermophilus)
[PDB|1Z8S] (DnaB binding domain, AA 452-597, Geobacillus stearothermophilus)
[PDB|4M4W] (the [protein|5CF09E089AA70A32F640CE0D33D1F05312AAE24D|dnaC]-[protein|AC1B507C6B1640E9BEA1F9F01628120F3680C976|dnaI]-[protein|F7B53E8AC92DAD15544C6208C2A5B41BABA73D6D|dnaG] complex) [Pubmed|24048025]
[AF|P05096]
Effectors of protein activity
the enzymatic activity is inhibited by (p)ppGpp during the stringent response, (p)ppGpp prevents DnaG from binding to replication forks and strongly inhibits primer extension [Pubmed|34389317,33504660,17350574]
[protein|F7B53E8AC92DAD15544C6208C2A5B41BABA73D6D|dnaG] primase activity is stimulated by [protein|5CF09E089AA70A32F640CE0D33D1F05312AAE24D|dnaC] [Pubmed|23563155]
[wiki|Localization]
Cytoplasm (Homogeneous) [Pubmed|16479537]
Expression and Regulation
Operons
genes
[gene|398924959D6C82918617C4B050F72D5E78EE12E9|yqxD]-[gene|F7B53E8AC92DAD15544C6208C2A5B41BABA73D6D|dnaG]-[gene|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]
description
[Pubmed|3298998]
regulation
enzymatic activity is inhibited by (p)ppGpp during the 'stringent response
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|3298998], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, [Pubmed|3127379], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]
additional information
An [wiki|ncRNA|antisense RNA] is predicted for [gene|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA] [PubMed|20525796]
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[gene|398924959D6C82918617C4B050F72D5E78EE12E9|yqxD]→[gene|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]

2024-03-14 00:02:57

TPed

131

345BE3439F4414F1DA2FD49B273925B808601AB7

E9F2D1769ACB2AF65C67D8854D4F58BC49368B55

References
Reviews
22797751,17350566,27149325,28075389
Original Publications
3919021,20122408,14651647,16479537,19415803,17350574,23563155,23268446,21419346,24048025,9849877,9722641,28386108,28575448,33504660,34389317

F7B53E8AC92DAD15544C6208C2A5B41BABA73D6D

Page visits: 3901

Time of last update: 2024-03-28 11:04:27

Author of last update: Jstuelk