Difference between revisions of "DnaE"

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(Expression and regulation)
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:'''  
+
* '''Operon:''' ''[[dnaE]]'' (according to [http://dbtbs.hgc.jp/COG/prom/dnaE.html DBTBS])
  
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
** repressed when no DNA damage is present ([[LexA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14593098,16267290 PubMed]
+
** induced by DNA damage ([[LexA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14593098,16267290 PubMed]
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 
** [[LexA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14593098,16267290 PubMed]
 
** [[LexA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14593098,16267290 PubMed]
  
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =

Revision as of 22:01, 18 December 2009

  • Description: DNA polymerase III (alpha subunit)

Gene name dnaE
Synonyms
Essential yes PubMed
Product DNA polymerase III (alpha subunit)
Function DNA replication
MW, pI 125 kDa, 6.02
Gene length, protein length 3345 bp, 1115 aa
Immediate neighbours ytsJ, ytrI
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
DnaE context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU29230

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
  • Protein family: DnaE subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References