dnaE

dnaE
168

lagging strand DNA polymerase III (alpha subunit), part of the [wiki|replisome]

locus
BSU_29230
Molecular weight
125.14 kDa
pI
6.02
Protein length
Gene length
function
[category|SW.3.1.1|DNA replication](lagging strand)
product
lagging strand DNA polymerase III (alpha subunit)
essential
yes
ec
2.7.7.7
synonyms
dnaE

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0587 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,991,269  2,994,616
Phenotypes of a mutant
essential [Pubmed|12682299]
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The protein
Catalyzed reaction/ biological activity
2'-deoxyribonucleoside 5'-triphosphate + DNA(n) --> diphosphate + DNA(n+1) (according to UniProt)
required for bacteriophage SPP1 replication [Pubmed|23268446]
Protein family
DNA polymerase type-C family (with [protein|48BC0FA9901F174472EB070C539EC75EC7C10E88|yorL] and [protein|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC], according to UniProt)
Structure
[PDB|3E0D] (from ''Thermus aquaticus'', 39% identity) [Pubmed|18691598]
[AF|O34623]
[wiki|Localization]
forms foci, the foci dissappear after the formation of DNA mismatches in a [protein|E2A8B04CD729332F9E6A13195181983F751F2F25|mutS]-dependent manner or after DNA replication arrest [Pubmed|21958350]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|65B5EA4DBDF5B260DED754B8A7B226AA88F17B55|dnaE]-[gene|45229A538D5124B60CE05DDC8699B9BC3D5AD9FF|ytsJ]
description
[pubmed|22383849]
regulation
induced by DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14593098,16267290 PubMed]
regulatory mechanism
[protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: repression, [PubMed|14593098,16267290], in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]
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[gene|65B5EA4DBDF5B260DED754B8A7B226AA88F17B55|dnaE]→[gene|45229A538D5124B60CE05DDC8699B9BC3D5AD9FF|ytsJ]

2024-03-23 19:21:51

Jstuelk

89

5807e5e50bd25f38dbc805171d3f14f5354cd973

F93681166D0A46D0BAA5D0678231F87A3D3BB25B

References
Reviews
22933559,28073967
Original Publications
11721055,14593098,16267290,20122408,23563155,23017159,21958350,23268446,24062730,24106089,18691598,28575448,28878042,30686488,31251806,33504660,37109494

65B5EA4DBDF5B260DED754B8A7B226AA88F17B55

Page visits: 5025

Time of last update: 2024-03-28 19:03:04

Author of last update: Jstuelk