DnaB

• Description: initiation of chromosome replication/ membrane attachment protein
  
  

Gene name	dnaB
Synonyms
Essential	yes PubMed
Product	initiation of chromosome replication/ membrane attachment protein
Function	DNA replication
MW, pI	54 kDa, 5.278
Gene length, protein length	1416 bp, 472 aa
Immediate neighbours	dnaI, ytcG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: DnaB-PriA, DnaB-DnaI, DnaB-DnaC, DnaD-DnaB, DnaB-DnaX PubMed

• Localization: Membrane-proximal (Spotty) PubMed forms foci, close to oriC PubMed

Database entries

• Structure:

• Swiss prot entry:

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Meile et al. (2006) Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory Proteomics 6: 2135-2146. PubMed
2. Haroniti et al. (2004) The clamp-loader-helicase interaction in Bacillus. Atomic force microscopy reveals the structural organisation of the DnaB-tau complex in Bacillus.J. Mol. Biol. 336: 381-393. PubMed
3. Imai et al. (2000) Subcellular localization of Dna-initiation proteins of Bacillus subtilis: evidence that chromosome replication begins at either edge of the nucleoids. Mol. Microbiol. 36: 1037-1048. PubMed