dltA

168

D-alanyl-D-alanine carrier protein ligase, alanylation of teichoic acid provides some resistance against positively charged antimicrobial peptides and against bacteriophage infection

locus: BSU_38500

Molecular weight: 55.64 kDa

pI: 4.93

Protein length: 503 aa

Gene length: 1512 bp

function: biosynthesis of teichoic acid

product: D-alanyl-D-alanine carrier protein ligase

essential: no

ec: 6.1.1.13

synonyms: dltA, ipa-5r, dae

List of homologs in different organisms, belongs to COG1020 (Galperin et al., 2021)

Gene

Coordinates: 3,952,275  3,953,786

Phenotypes of a mutant

• increased sensitivity to lysozyme [Pubmed|21856855]
• more sensitive to nisin [Pubmed|23980836]

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The protein

Catalyzed reaction/ biological activity

• ATP + D-alanine + holo-[protein|862D977CF1E185597329A1EF0915485F72482A03|dltC] --> AMP + D-alanyl-[protein|862D977CF1E185597329A1EF0915485F72482A03|dltC] + diphosphate (according to UniProt) [pubmed|37308592]

Protein family

• [wiki|ATP-dependent AMP-binding enzyme family] (according to UniProt)

Structure

• [PDB|3E7X]
• [AF|P39581]

[wiki|Localization]

• cytoplasm (according to UniProt)

Biological materials

Mutant

• BKE38500 ([gene|795CD64B65D7CBF26E5F706C7617196CC6FCF864|dltA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38500 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTCATAGTTATTCTCTCTC,  downstream forward: _UP4_CGCATTGGCGAAGAGGTTCT
• BKK38500 ([gene|795CD64B65D7CBF26E5F706C7617196CC6FCF864|dltA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38500 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTCATAGTTATTCTCTCTC,  downstream forward: _UP4_CGCATTGGCGAAGAGGTTCT

labs

• [wiki|Mohamed Marahiel], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]

References

Reviews

• 24819367

Original Publications

• 14762009,15955059,18784082,23980836,7797557,19324056,16306698,21856855,21926231,29069433,29794222,30283133,34878184,37308592