Difference between revisions of "DgkB"

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(Biological materials)
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* '''Mutant:'''
 
* '''Mutant:'''
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** GP1391 ''dgkB::cat'', available in [[Jörg Stülke]]'s lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 16:05, 13 August 2013

  • Description: diacylglycerol kinase

Gene name dgkB
Synonyms yerQ
Essential yes PubMed
Product diacylglycerol kinase
Function biosynthesis of lipoteichoic acid
Gene expression levels in SubtiExpress: dgkB
MW, pI 33 kDa, 5.635
Gene length, protein length 909 bp, 303 aa
Immediate neighbours yerP, rlmCD
Sequences Protein DNA DNA_with_flanks
Genetic context
YerQ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
DgkB expression.png
























Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU06720

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): BmrU

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3S40 (from B. anthracis, 32% identity, 68% similarity)
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Satoshi Matsuoka, Michihiro Hashimoto, Yusuke Kamiya, Takeshi Miyazawa, Kazuki Ishikawa, Hiroshi Hara, Kouji Matsumoto
The Bacillus subtilis essential gene dgkB is dispensable in mutants with defective lipoteichoic acid synthesis.
Genes Genet Syst: 2011, 86(6);365-76
[PubMed:22451476] [WorldCat.org] [DOI] (I p)

Agoston Jerga, Ying-Jie Lu, Gustavo E Schujman, Diego de Mendoza, Charles O Rock
Identification of a soluble diacylglycerol kinase required for lipoteichoic acid production in Bacillus subtilis.
J Biol Chem: 2007, 282(30);21738-45
[PubMed:17535816] [WorldCat.org] [DOI] (P p)