DgcW

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  • Description: diguanylate cyclase and potential phosphodiesterase

Gene name dgcW
Synonyms ykoW
Essential no
Product diguanylate cyclase and potential phosphodiesterase
Function synthesis of c-di-GMP
Gene expression levels in SubtiExpress: dgcW
MW, pI 84 kDa, 6.823
Gene length, protein length 2247 bp, 749 aa
Immediate neighbours ykoV, ykoX
Sequences Protein DNA DNA_with_flanks
Genetic context
YkoW context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YkoW expression.png















Categories containing this gene/protein

membrane proteins, metabolism of signalling nucleotides

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU13420

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • synthesis of c-di-GMP from two molecules of GTP PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • contains a N-terminal PAS domain PubMed
    • contains a central GGDEF domain and a C-terminal EAL domain PubMed
    • aa 290-735 are similar to E. coli CsrD (18% identity, 43% similarity)
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Xiaohui Gao, Sampriti Mukherjee, Paige M Matthews, Loubna A Hammad, Daniel B Kearns, Charles E Dann
Functional characterization of core components of the Bacillus subtilis cyclic-di-GMP signaling pathway.
J Bacteriol: 2013, 195(21);4782-92
[PubMed:23893111] [WorldCat.org] [DOI] (I p)

Yun Chen, Yunrong Chai, Jian-hua Guo, Richard Losick
Evidence for cyclic Di-GMP-mediated signaling in Bacillus subtilis.
J Bacteriol: 2012, 194(18);5080-90
[PubMed:22821967] [WorldCat.org] [DOI] (I p)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Kazushi Suzuki, Paul Babitzke, Sidney R Kushner, Tony Romeo
Identification of a novel regulatory protein (CsrD) that targets the global regulatory RNAs CsrB and CsrC for degradation by RNase E.
Genes Dev: 2006, 20(18);2605-17
[PubMed:16980588] [WorldCat.org] [DOI] (P p)

M Y Galperin, T A Gaidenko, A Y Mulkidjanian, M Nakano, C W Price
MHYT, a new integral membrane sensor domain.
FEMS Microbiol Lett: 2001, 205(1);17-23
[PubMed:11728710] [WorldCat.org] [DOI] (P p)