Difference between revisions of "DgcW"

From SubtiWiki
Jump to: navigation, search
(Biological materials)
(Biological materials)
Line 120: Line 120:
 
* '''Mutant:'''
 
* '''Mutant:'''
 
** GP850 (''[[ykoW]]''::''cat''), available in [[Stülke]] lab
 
** GP850 (''[[ykoW]]''::''cat''), available in [[Stülke]] lab
 
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 07:23, 19 April 2013

  • Description: potential diguanylate cyclase and phosphodiesterase

Gene name ykoW
Synonyms
Essential no
Product potential diguanylate cyclase and phosphodiesterase
Function unknown
Gene expression levels in SubtiExpress: ykoW
MW, pI 84 kDa, 6.823
Gene length, protein length 2247 bp, 749 aa
Immediate neighbours ykoV, ykoX
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YkoW context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YkoW expression.png















Categories containing this gene/protein

membrane proteins, metabolism of signalling nucleotides

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU13420

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • contains a central GGDEF domain and a C-terminal EAL domain PubMed
    • aa 290-735 are similar to E. coli CsrD (18% identity, 43% similarity)
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Kazushi Suzuki, Paul Babitzke, Sidney R Kushner, Tony Romeo
Identification of a novel regulatory protein (CsrD) that targets the global regulatory RNAs CsrB and CsrC for degradation by RNase E.
Genes Dev: 2006, 20(18);2605-17
[PubMed:16980588] [WorldCat.org] [DOI] (P p)

M Y Galperin, T A Gaidenko, A Y Mulkidjanian, M Nakano, C W Price
MHYT, a new integral membrane sensor domain.
FEMS Microbiol Lett: 2001, 205(1);17-23
[PubMed:11728710] [WorldCat.org] [DOI] (P p)