DfrA

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  • Description: dihydrofolate reductase

Gene name dfrA
Synonyms tmp
Essential yes PubMed
Product dihydrofolate reductase
Function biosynthesis of folate
Interactions involving this protein in SubtInteract: DfrA
Metabolic function and regulation of this protein in SubtiPathways:
Nucleotides (regulation), Folate
MW, pI 19 kDa, 5.219
Gene length, protein length 504 bp, 168 aa
Immediate neighbours ypkP, thyB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
DfrA context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

biosynthesis of cofactors, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU21810

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH (according to Swiss-Prot)
  • Protein family: spmB family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1ZDR (Geobacillus stearothermophilus)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

M Iwakura, M Kawata, K Tsuda, T Tanaka
Nucleotide sequence of the thymidylate synthase B and dihydrofolate reductase genes contained in one Bacillus subtilis operon.
Gene: 1988, 64(1);9-20
[PubMed:2840350] [WorldCat.org] [DOI] (P p)

T T Myoda, V L Funanage
Coregulation of dihydrofolate reductase and thymidylate synthase B in bacillus subtilis.
Biochim Biophys Acta: 1985, 824(2);99-103
[PubMed:3918568] [WorldCat.org] [DOI] (P p)

T T Myoda, S V Lowther, V L Funanage, F E Young
Cloning and mapping of the dihydrofolate reductase gene of Bacillus subtilis.
Gene: 1984, 29(1-2);135-43
[PubMed:6436144] [WorldCat.org] [DOI] (P p)