Difference between revisions of "DfrA"

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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=DfrA DfrA]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=DfrA DfrA]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)], [http://subtiwiki.uni-goettingen.de/pathways/folate_biosynthesis.html Folate]'''
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=dfrA dfrA]'''
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 5.219   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 5.219   

Revision as of 11:02, 7 January 2014

  • Description: dihydrofolate reductase

Gene name dfrA
Synonyms tmp
Essential yes PubMed
Product dihydrofolate reductase
Function biosynthesis of folate
Gene expression levels in SubtiExpress: dfrA
Interactions involving this protein in SubtInteract: DfrA
Metabolic function and regulation of this protein in SubtiPathways:
dfrA
MW, pI 19 kDa, 5.219
Gene length, protein length 504 bp, 168 aa
Immediate neighbours ypkP, thyB
Sequences Protein DNA DNA_with_flanks
Genetic context
DfrA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
DfrA expression.png















Categories containing this gene/protein

biosynthesis of cofactors, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU21810

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH (according to Swiss-Prot)
  • Protein family: spmB family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1ZDR (Geobacillus stearothermophilus)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Kevin Francis, Vanja Stojkovic, Amnon Kohen
Preservation of protein dynamics in dihydrofolate reductase evolution.
J Biol Chem: 2013, 288(50);35961-8
[PubMed:24158440] [WorldCat.org] [DOI] (I p)

M Iwakura, M Kawata, K Tsuda, T Tanaka
Nucleotide sequence of the thymidylate synthase B and dihydrofolate reductase genes contained in one Bacillus subtilis operon.
Gene: 1988, 64(1);9-20
[PubMed:2840350] [WorldCat.org] [DOI] (P p)

T T Myoda, V L Funanage
Coregulation of dihydrofolate reductase and thymidylate synthase B in bacillus subtilis.
Biochim Biophys Acta: 1985, 824(2);99-103
[PubMed:3918568] [WorldCat.org] [DOI] (P p)

T T Myoda, S V Lowther, V L Funanage, F E Young
Cloning and mapping of the dihydrofolate reductase gene of Bacillus subtilis.
Gene: 1984, 29(1-2);135-43
[PubMed:6436144] [WorldCat.org] [DOI] (P p)