DeoD

• Description: purine nucleoside phosphorylase
  
  

Gene name	deoD
Synonyms
Essential	no
Product	purine nucleoside phosphorylase
Function	purine salvage and interconversion
Gene expression levels in SubtiExpress: deoD
Metabolic function and regulation of this protein in SubtiPathways: Purine salvage, Nucleoside catabolism
MW, pI	25 kDa, 4.978
Gene length, protein length	699 bp, 233 aa
Immediate neighbours	yodJ, yoyE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, membrane proteins

The gene

Basic information

• Locus tag: BSU19630

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • forms hexamers PubMed

• Localization:
  • membrane (according to Swiss-Prot)

Database entries

• Structure: 4D8V PubMed

• UniProt: O34925

• KEGG entry: [2]

• E.C. number: 2.4.2.1

Expression and regulation

• Operon:

• Expression browser: deoD PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Priscila O de Giuseppe, Nadia H Martins, Andreia N Meza, Camila R dos Santos, Humberto D'Muniz Pereira, Mario T Murakami
Insights into phosphate cooperativity and influence of substrate modifications on binding and catalysis of hexameric purine nucleoside phosphorylases.
PLoS One: 2012, 7(9);e44282
[PubMed:22957058] [WorldCat.org] [DOI] (I p)

Haojian Li, Guoqiang Zhang, Aihua Deng, Ning Chen, Tingyi Wen
De novo engineering and metabolic flux analysis of inosine biosynthesis in Bacillus subtilis.
Biotechnol Lett: 2011, 33(8);1575-80
[PubMed:21424839] [WorldCat.org] [DOI] (I p)

Rosa Grenha, Vladimir M Levdikov, Mark J Fogg, Elena V Blagova, James A Brannigan, Anthony J Wilkinson, Keith S Wilson
Structure of purine nucleoside phosphorylase (DeoD) from Bacillus anthracis.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2005, 61(Pt 5);459-62
[PubMed:16511068] [WorldCat.org] [DOI] (I p)