Difference between revisions of "DefB"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykrA]]'', ''[[ykzV]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykrA]]'', ''[[ykzV]]''
 
|-
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU14560 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU14560 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU14560 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU14560 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU14560 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU14560 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:ykrB_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ykrB_context.gif]]

Revision as of 10:06, 14 May 2013

  • Description: major formylmethionine deformylase

Gene name ykrB
Synonyms defB
Essential no
Product formylmethionine deformylase
Function translation
Gene expression levels in SubtiExpress: ykrB
MW, pI 20 kDa, 5.456
Gene length, protein length 552 bp, 184 aa
Immediate neighbours ykrA, ykzV
Sequences Protein DNA DNA_with_flanks
Genetic context
YkrB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YkrB expression.png















Categories containing this gene/protein

translation, protein modification

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU14560

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Formyl-L-methionyl peptide + H2O = formate + methionyl peptide (according to Swiss-Prot)
  • Protein family: polypeptide deformylase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1LQY (complex with antibiotic actinonin, Geobacillus stearothermophilus)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Michael Haas, Dieter Beyer, Reinhold Gahlmann, Christoph Freiberg
YkrB is the main peptide deformylase in Bacillus subtilis, a eubacterium containing two functional peptide deformylases.
Microbiology (Reading): 2001, 147(Pt 7);1783-1791
[PubMed:11429456] [WorldCat.org] [DOI] (P p)