Difference between revisions of "DeaD"

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(Labs working on this gene/protein)
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=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
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[[Dagmar Klostermeier]], Biozentrum Basel, Switzerland [http://www.biozentrum.unibas.ch/klostermeier/index.html homepage]
  
 
=Your additional remarks=
 
=Your additional remarks=

Revision as of 09:00, 8 April 2009

  • Description: DEAD-box RNA helicase

Gene name deaD
Synonyms yxiN
Essential no
Product ATP-dependent RNA helicase
Function RNA helicase
MW, pI 53 kDa, 7.706
Gene length, protein length 1437 bp, 479 aa
Immediate neighbours yxiO, yxiM
Gene sequence (+200bp) Protein sequence
Caution: The sequence for this gene in SubtiList contains errors
Genetic context
DeaD context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: RNA helicase
  • Protein family: DEAD-box RNA helicase

Extended information on the protein

  • Kinetic information:
  • Domains:
    • Helicase domain 1: AS 1-203
    • Linker region: AS 204-212
    • Helicase domain 2: AS 207-368
    • RNA-binding domain: AS 404-479
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1121 (tet), available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Dagmar Klostermeier, Biozentrum Basel, Switzerland homepage

Your additional remarks

References

  1. Caruthers, J. M., Hu, Y. & McKay, D. B. (2006). Structure of the second domain of the Bacillus subtilis DEAD-box RNA helicase YxiN. Acta Crystallogr Sect F Struct Biol Cryst Commun 62, 1191-1195.PubMed
  2. Karginov, F. V., Caruthers, J. M., Hu, Y., McKay, D. B. & Uhlenbeck, O. C. (2005). YxiN is a modular protein combining a DEx(D/H) core and a specific RNA-binding domain. J Biol Chem 280, 35499-35505.PubMed
  3. Karow, A. R., Theissen, B. & Klostermeier, D. (2007). Authentic interdomain communication in an RNA helicase reconstituted by expressed protein ligation of two helicase domains. FEBS J 274, 463-473.PubMed
  4. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed