Dat

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  • Description: D-alanine aminotransferase

(Another gene coding for O6-methylguanine DNA alkyltransferase was formely known as dat, it is now called ogt)

Gene name dat
Synonyms yheM
Essential no
Product D-alanine aminotransferase
Function peptidoglycan precursor biosynthesis
MW, pI 31 kDa, 4.871
Gene length, protein length 846 bp, 282 aa
Immediate neighbours yheN, nhaC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Dat context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-alanine + 2-oxoglutarate = pyruvate + D-glutamate (according to Swiss-Prot)
  • Protein family: class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 1G2W (E177S Mutant, Geobacillus stearothermophilus, 43% identity)

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed