Dat

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(Another gene coding for O6-methylguanine DNA alkyltransferase was formely known as dat, it is now called ogt)

Gene name dat
Synonyms yheM
Essential no
Product D-alanine aminotransferase
Function peptidoglycan precursor biosynthesis
Gene expression levels in SubtiExpress: dat
Metabolic function and regulation of this protein in SubtiPathways:
Cell wall
MW, pI 31 kDa, 4.871
Gene length, protein length 846 bp, 282 aa
Immediate neighbours yheN, nhaC
Sequences Protein DNA Advanced_DNA
Genetic context
Dat context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Dat expression.png




























Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU09670

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-alanine + 2-oxoglutarate = pyruvate + D-glutamate (according to Swiss-Prot)
  • Protein family: class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1G2W (E177S Mutant, Geobacillus stearothermophilus, 43% identity)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Le Thi Tam, Christine Eymann, Haike Antelmann, Dirk Albrecht, Michael Hecker
Global gene expression profiling of Bacillus subtilis in response to ammonium and tryptophan starvation as revealed by transcriptome and proteome analysis.
J Mol Microbiol Biotechnol: 2007, 12(1-2);121-30
[PubMed:17183219] [WorldCat.org] [DOI] (P p)

P P Taylor, I G Fotheringham
Nucleotide sequence of the Bacillus licheniformis ATCC 10716 dat gene and comparison of the predicted amino acid sequence with those of other bacterial species.
Biochim Biophys Acta: 1997, 1350(1);38-40
[PubMed:9003455] [WorldCat.org] [DOI] (P p)