Difference between revisions of "DapI"

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m (Jstuelk moved page YkuR to DapI: 24261876)
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|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of lysine and peptidoglycan
 
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of lysine and peptidoglycan
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14190 ykuR]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14190 dapI]
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/lys_threo.html Lys, Thr]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/lys_threo.html Lys, Thr]'''
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' N-acetyl-LL-2,6-diaminoheptanedioate + H<sub>2</sub>O = acetate + LL-2,6-diaminoheptanedioate (according to Swiss-Prot)
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* '''Catalyzed reaction/ biological activity:''' N-acetyl-LL-2,6-diaminopimelate + H<sub>2</sub>O --> acetate + LL-2,6-diaminopimelate {{PubMed|24261876}}
  
 
* '''Protein family:''' peptidase M20A family (according to Swiss-Prot)
 
* '''Protein family:''' peptidase M20A family (according to Swiss-Prot)
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
+
* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''
+
* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
 
* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
 +
** [[DapI]]-[[MreB]] {{PubMed|24261876}}
  
 
* '''[[Localization]]:'''
 
* '''[[Localization]]:'''
 +
** forms uniformly distributed foci in the cytoplasm {{PubMed|24261876}}
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Additional information:'''  
 
* '''Additional information:'''  
The gene is annotated in KEGG as an ortholog of N-acetyldiaminopimelate deacetylase EC 3.5.1.47. In MetaCyc it is marked as “similar to hippurate hydrolase”. No EC annotation is available in Swiss-Prot. No literature/experimental evidence supporting the annotation is available. {{PubMed|19935659}}
 
  
 
=Biological materials =
 
=Biological materials =
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=References=
 
=References=
 
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<pubmed>24261876 </pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 12:01, 5 January 2014

  • Description: N-acetyl-diaminopimelate deacetylase

Gene name ykuR
Synonyms dapL
Essential yes PubMed
Product N-acetyl-diaminopimelate deacetylase
Function biosynthesis of lysine and peptidoglycan
Gene expression levels in SubtiExpress: dapI
Metabolic function and regulation of this protein in SubtiPathways:
Lys, Thr
MW, pI 41 kDa, 5.102
Gene length, protein length 1122 bp, 374 aa
Immediate neighbours ykuQ, ykuS
Sequences Protein DNA DNA_with_flanks
Genetic context
YkuR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YkuR expression.png















Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, biosynthesis/ acquisition of amino acids, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU14190

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: N-acetyl-LL-2,6-diaminopimelate + H2O --> acetate + LL-2,6-diaminopimelate PubMed
  • Protein family: peptidase M20A family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Anne-Stéphanie Rueff, Arnaud Chastanet, Julia Domínguez-Escobar, Zhizhong Yao, James Yates, Maria-Victoria Prejean, Olivier Delumeau, Philippe Noirot, Roland Wedlich-Söldner, Sergio R Filipe, Rut Carballido-López
An early cytoplasmic step of peptidoglycan synthesis is associated to MreB in Bacillus subtilis.
Mol Microbiol: 2014, 91(2);348-62
[PubMed:24261876] [WorldCat.org] [DOI] (I p)