Difference between revisions of "DapF"

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|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of lysine and peptidoglycan  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of lysine and peptidoglycan  
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU32170 dapF]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU32170 dapF]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/lys_threo.html Lys, Thr]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/lys_threo.html Lys, Thr]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sufA]]'', ''[[yutK]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sufA]]'', ''[[yutK]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15207&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU32170 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU32170 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU32170 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:dapF_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:dapF_context.gif]]

Revision as of 13:46, 13 May 2013

  • Description: diaminopimelate epimerase

Gene name dapF
Synonyms yutL
Essential yes PubMed
Product diaminopimelate epimerase
Function biosynthesis of lysine and peptidoglycan
Gene expression levels in SubtiExpress: dapF
Metabolic function and regulation of this protein in SubtiPathways:
Lys, Thr
MW, pI 30 kDa, 4.506
Gene length, protein length 852 bp, 284 aa
Immediate neighbours sufA, yutK
Sequences Protein DNA Advanced_DNA
Genetic context
DapF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
DapF expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU32170

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate (according to Swiss-Prot)
  • Protein family: diaminopimelate epimerase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2OTN (from Bacillus anthracis str. ames, 62% identity, 75% similarity)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References