DapA

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  • Description: dihydrodipicolinate synthase

Gene name dapA
Synonyms
Essential yes PubMed
Product dihydrodipicolinate synthase
Function biosynthesis of lysine and peptidoglycan
Gene expression levels in SubtiExpress: dapA
Metabolic function and regulation of this protein in SubtiPathways:
Lys, Thr
MW, pI 30 kDa, 4.928
Gene length, protein length 870 bp, 290 aa
Immediate neighbours dapG, rnjB
Sequences Protein DNA Advanced_DNA
Genetic context
DapA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
DapA expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, sporulation proteins, essential genes

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

  • Locus tag: BSU16770

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O (according to Swiss-Prot)
  • Protein family: DHDPS family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3CPR (from Corynebacterium glutamicum, 39% identity, 58% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

R A Daniel, J Errington
Cloning, DNA sequence, functional analysis and transcriptional regulation of the genes encoding dipicolinic acid synthetase required for sporulation in Bacillus subtilis.
J Mol Biol: 1993, 232(2);468-83
[PubMed:8345520] [WorldCat.org] [DOI] (P p)

N Y Chen, S Q Jiang, D A Klein, H Paulus
Organization and nucleotide sequence of the Bacillus subtilis diaminopimelate operon, a cluster of genes encoding the first three enzymes of diaminopimelate synthesis and dipicolinate synthase.
J Biol Chem: 1993, 268(13);9448-65
[PubMed:8098035] [WorldCat.org] (P p)