dacF

168

Class C penicillin-binding protein I, D-alanyl-D-alanine carboxypeptidase

locus: BSU_23480

Molecular weight: 43.13 kDa

pI: 9.91

Protein length: 389 aa

Gene length: 1170 bp

function: control of peptide cross-linking in spore peptidoglycan

product: penicillin-binding protein I, D-alanyl-D-alanine carboxypeptidase

essential: no

synonyms: dacF

List of homologs in different organisms, belongs to COG1686 (Galperin et al., 2021)

Gene

Coordinates: 2,445,094 → 2,446,263

Phenotypes of a mutant

• the mutant tends to acquire suppressor mutations that result in improved growth and colony morphology [Pubmed|28189581]

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The protein

Catalyzed reaction/ biological activity

• modifies degree of cross-linking of glycan strands in peptidoglycan [pubmed|9864321]
• Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine (according to UniProt)

Protein family

• [wiki|Peptidase S11 family] (according to UniProt)

Structure

• [PDB|4K91] (from Pseudomonas aeruginosa, 38% identity) [pubmed|23629710]
• [AF|P38422]

Paralogous protein(s)

• [protein|7C3081BC8D416127A881627EB56C0628359111CF|dacA], [protein|76825D77907E8CE47B17ED56D7E2A348B1ADA5EC|dacB]

[wiki|Localization]

• secreted (via signal peptide) (according to UniProt)

Biological materials

Mutant

• BKE23480 (Δ[gene|F7AD78F9AB98A150E8CDFC1D01A9F938FF9F8BD1|dacF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23480 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTCAAAAGCCCTCCATT,  downstream forward: _UP4_TAATTATGCCGAATGACCAC
• BKK23480 (Δ[gene|F7AD78F9AB98A150E8CDFC1D01A9F938FF9F8BD1|dacF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23480 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTCAAAAGCCCTCCATT,  downstream forward: _UP4_TAATTATGCCGAATGACCAC

References

Reviews

• 18266855,33709487,35638784

Original Publications

• 16497325,8936302,9864321,12107147,20817675,22123250,23629710,32419322