Difference between revisions of "DacC"

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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1473 bp, 491 aa  
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1473 bp, 491 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ppsA]]'', ''[[yoxA]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ppsA]]'', ''[[galM]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13718&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13718&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala (according to Swiss-Prot)
+
* '''Catalyzed reaction/ biological activity:''' cleavage ofD-Ala-D-Ala interpeptide bridges in peptidoglycan {{PubMed|19758330}}
  
* '''Protein family:''' ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)
+
* '''Protein family:'''
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:'''  
+
* '''Operon:''' ''[[galM]]-[[dacC]]'' {{PubMed|9733705}}
  
* '''[[Sigma factor]]:'''  
+
* '''[[Sigma factor]]:''' [[SigH]] {{PubMed|9733705}}, [[SigB]] {{PubMed|10482513}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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=References=
 
=References=
 
+
<pubmed> 9733705 10482513 19758330 14731276,,9864321, </pubmed>
<pubmed>9733705,14731276,,9864321, </pubmed>
 
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 10:48, 18 September 2009

  • Description: penicillin-binding protein 4A, D-alanyl-D-alanine carboxypeptidase

Gene name dacC
Synonyms pbp
Essential no
Product penicillin-binding protein 4A,
D-alanyl-D-alanine carboxypeptidase
Function carboxypeptidase
MW, pI 52 kDa, 5.413
Gene length, protein length 1473 bp, 491 aa
Immediate neighbours ppsA, galM
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
DacC context.gif
This image was kindly provided by SubtiList




The gene

Basic information

  • Locus tag: BSU18350

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: cleavage ofD-Ala-D-Ala interpeptide bridges in peptidoglycan PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 1W5D, 2J9P (complex with a peptidoglycan mimetic peptide)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Colette Duez, Astrid Zervosen, Nathalie Teller, Rémy Melkonian, Emmanuel Banzubazé, Fabrice Bouillenne, André Luxen, Jean-Marie Frère
Characterization of the proteins encoded by the Bacillus subtilis yoxA-dacC operon.
FEMS Microbiol Lett: 2009, 300(1);42-7
[PubMed:19758330] [WorldCat.org] [DOI] (I p)

Dirk-Jan Scheffers, Laura J F Jones, Jeffery Errington
Several distinct localization patterns for penicillin-binding proteins in Bacillus subtilis.
Mol Microbiol: 2004, 51(3);749-64
[PubMed:14731276] [WorldCat.org] [DOI] (P p)

A Petersohn, J Bernhardt, U Gerth, D Höper, T Koburger, U Völker, M Hecker
Identification of sigma(B)-dependent genes in Bacillus subtilis using a promoter consensus-directed search and oligonucleotide hybridization.
J Bacteriol: 1999, 181(18);5718-24
[PubMed:10482513] [WorldCat.org] [DOI] (P p)

D L Popham, M E Gilmore, P Setlow
Roles of low-molecular-weight penicillin-binding proteins in Bacillus subtilis spore peptidoglycan synthesis and spore properties.
J Bacteriol: 1999, 181(1);126-32
[PubMed:9864321] [WorldCat.org] [DOI] (P p)

L B Pedersen, T Murray, D L Popham, P Setlow
Characterization of dacC, which encodes a new low-molecular-weight penicillin-binding protein in Bacillus subtilis.
J Bacteriol: 1998, 180(18);4967-73
[PubMed:9733705] [WorldCat.org] [DOI] (P p)