DacA

• Description: penicillin-binding protein 5*, D-alanyl-D-alanine carboxypeptidase
  
  

Gene name	dacA
Synonyms
Essential	no
Product	penicillin-binding protein 5*, D-alanyl-D-alanine carboxypeptidase
Function	carboxypeptidase
Gene expression levels in SubtiExpress: dacA
MW, pI	48 kDa, 5.668
Gene length, protein length	1329 bp, 443 aa
Immediate neighbours	guaB, pdxS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, membrane proteins

The gene

Basic information

• Locus tag: BSU00100

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala (according to Swiss-Prot)

• Protein family: peptidase S11 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • secreted (according to Swiss-Prot), membrane associated PubMed

Database entries

• Structure:

• UniProt: P08750

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: dacA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A742 ( dacA::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

D L Popham, M E Gilmore, P Setlow
Roles of low-molecular-weight penicillin-binding proteins in Bacillus subtilis spore peptidoglycan synthesis and spore properties.
J Bacteriol: 1999, 181(1);126-32
[PubMed:9864321] [WorldCat.org] [DOI] (P p)