Difference between revisions of "CysS"

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(References)
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|style="background:#ABCDEF;" align="center"|'''Function''' || translation  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || translation  
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU00940 cysS]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00940 cysS]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/tRNA_charging.html tRNA charging], [http://subtiwiki.uni-goettingen.de/pathways/cys_meth_and_sulfate_assimilation.html Cys, Met & Sulfate assimilation]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/tRNA_charging.html tRNA charging], [http://subtiwiki.uni-goettingen.de/pathways/cys_meth_and_sulfate_assimilation.html Cys, Met & Sulfate assimilation]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cysE]]'', ''[[mrnC]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cysE]]'', ''[[mrnC]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11870&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00940 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00940 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00940 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:cysS_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:cysS_context.gif]]

Revision as of 11:57, 13 May 2013

  • Description: cysteine-tRNA synthetase

Gene name cysS
Synonyms snpA
Essential yes PubMed
Product cysteine-tRNA synthetase
Function translation
Gene expression levels in SubtiExpress: cysS
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging, Cys, Met & Sulfate assimilation
MW, pI 53 kDa, 5.07
Gene length, protein length 1398 bp, 466 aa
Immediate neighbours cysE, mrnC
Sequences Protein DNA Advanced_DNA
Genetic context
CysS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CysS expression.png















Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box

The gene

Basic information

  • Locus tag: BSU00940

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys) (according to Swiss-Prot)
  • Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Ser-270 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1U0B (from Escherichia coli, 42% identity, 56% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism: T-box, overlaps a transcription terminator upstream of cysE: transcription antitermination PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

Y Gagnon, R Breton, H Putzer, M Pelchat, M Grunberg-Manago, J Lapointe
Clustering and co-transcription of the Bacillus subtilis genes encoding the aminoacyl-tRNA synthetases specific for glutamate and for cysteine and the first enzyme for cysteine biosynthesis.
J Biol Chem: 1994, 269(10);7473-82
[PubMed:7510287] [WorldCat.org] (P p)